| 几种沙棘叶片内过氧化物酶同工酶酶谱和酶活性比较 |
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| 引用本文： | 何士敏,向邓云,李昌满.几种沙棘叶片内过氧化物酶同工酶酶谱和酶活性比较[J].安徽农业科学,2009(2):525-526. |
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| 作者姓名： | 何士敏 向邓云 李昌满 |
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| 作者单位： | 长江师范学院生命科学系,重庆涪陵,408100;长江师范学院生命科学系,重庆涪陵,408100;长江师范学院生命科学系,重庆涪陵,408100 |
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| 基金项目： | 长江师范学院重点扶持学科资助项目 |
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| 摘 要： | 目的]为沙棘的选种、开发和利用方面提供参考依据。方法]以3个沙棘品种（齐棘Ⅰ号、阿列依、楚伊）的嫩叶为材料，测定了它们的POD酶谱和酶活性。结果]酶谱带染色结果表明，齐棘Ⅰ号酶活性最强，阿列依次之，楚伊较弱。齐棘I号的POD同工酶酶谱带最多，共有9条带，其中深色带5条。阿列依具有4条酶带，其中深色带2条。楚伊具有3条酶带，其中深色带1条。△OD／（min·g）值愈大则酶活性愈强。齐棘Ⅰ号、阿列依、楚伊的△OD／（min·g）值分别为0．112、0．100和0．092。结论]供试的3种沙棘POD活性顺序为：齐棘Ⅰ号〉阿列依〉楚伊．其抗逆性由强至弱依次为齐棘Ⅰ号〉阿列依〉楚伊。
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| 关 键 词： | 沙棘 过氧化物酶 酶谱 抗逆性 |
Comparision of Peroxidase Isozyme Zymogram and Enzymatic Activity in Several Kinds of Hippophaes rhamnoides L.Leaves |
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| HE Shi-min et al.Comparision of Peroxidase Isozyme Zymogram and Enzymatic Activity in Several Kinds of Hippophaes rhamnoides L.Leaves[J].Journal of Anhui Agricultural Sciences,2009(2):525-526. |
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| Authors: | HE Shi-min |
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| Institution: | HE Shi-min et al(Department of Life Science,Changjiang Normal College,Fuling,Chongqing 408100) |
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| Abstract: | Objective] The objective was to provide the reference basis for the breeding,development and utilization of Hippophaes rhamnoides L.Method] With the tender leaves of 3 H.rhamnoides varieties(Qiji 1,Alieyi,Chuyi) as materials,their POD zymogram and activity of peroxidase were determined.Result] The result of staining zymogram showed that the enzyme activity of Qiji 1 was the strongest,the following was that of Alieyi and that of Chuyi was the lowest.POD isozyme zymogram bands of Qiji 1 was the most,being ... |
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| Keywords: | Hippophaes rhamnoides L Peroxidase Zymogram Stress resistance |
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