| 棉花优良品种中棉所19高频体细胞胚胎发生细胞系筛选与植株再生 |
| |
| 引用本文： | 张宝红,刘方,刘志红,巩万奎,姚长兵.棉花优良品种中棉所19高频体细胞胚胎发生细胞系筛选与植株再生[J].作物学报,2000,26(2):239-242. |
| |
| 作者姓名： | 张宝红 刘方 刘志红 巩万奎 姚长兵 |
| |
| 作者单位： | 中国农业科学院棉花研究所， 农业部棉花遗传改良重点开放实验室， 河南安阳， 455112 |
| |
| 基金项目： | 农业部重点科研计划高新技术与基础研究专题资助项目 |
| |
| 摘 要： | 选用我国优良棉花品种中棉所19进行组织培养， 研究了该品种的体细胞胚胎发生和植株再生能力。 中棉所19的体细胞胚胎发生有两种情况： 一是由外植体直接诱导获得胚性愈伤组织和体细胞胚； 二是先诱导获得愈伤组织， 再经继代培养获得胚胎发生。 单独使用ZT可在早期诱导胚胎发生， 在该情况下， 对子叶的诱导能力最强， 胚根
|
| 关 键 词： | 棉花 中棉所19 胚胎发生 植株再生 |
| 收稿时间： | 1998-06-25 |
| 修稿时间： | 1998-12-16 |
High Frequency Somatic Embryogenesis and Plant Regeneration of Elite Cotton Variety CCRI 19 |
| |
| ZHANG Bao,Hong,LIU Fang,LIU Zhi,Hong,GONG Wan,Kui,YAO Chang,Bing.High Frequency Somatic Embryogenesis and Plant Regeneration of Elite Cotton Variety CCRI 19[J].Acta Agronomica Sinica,2000,26(2):239-242. |
| |
| Authors: | ZHANG Bao Hong LIU Fang LIU Zhi Hong GONG Wan Kui YAO Chang Bing |
| |
| Institution: | Cotton Research Institute of CAAS, Key Lab. of Cotton Genetic Impro., Agri. Ministry PRC, Anyang Henan 455112 |
| |
| Abstract: | Chinese elite cotton variety CCRI 19 was chosen for tissue culture, and obtained callus with high frequency embryogenesis and somatic embryos. Embryogenic callus can produced directly from cotyledon, hypocotyle and radical, or from callus producing from explants. Embryogenic callus and somatic embryos could be directly obtained on the medium only with ZT, but it was influenced by explant sources. Cotyledon was easiest to be induced embryogenesis, and hypocotyl was the most difficult. The initial embryogenic callus was difficult to produce somatic embryos, but it could be improved by subculture on the medium with 0.1 mg/L ZT (or 2iP) and 2 g/L active carbon. Many abnormal embryos existed. About 20%~30% somatic embryos can be converted into plantlets on suitable medium This paper suggested an efficient procedure for CCRI 19 somatic embryogenesis and plant regeneration. |
| |
| Keywords: | Gossypium hirsutum L CCRI 19 Somatic embryogenesis Plant regeneration |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ |
| 点击此处可从《作物学报》浏览原始摘要信息 |
| 点击此处可从《作物学报》下载免费的PDF全文 |
|
