The stability of glutathione peroxidase activity in plasma from cattle,pigs and sheep on storage in the presence and absence of glutathione |
| |
| Authors: | W B Davidson D G Kennedy P J Hughes W J Blanchflower |
| |
| Institution: | (1) Veterinary Research Laboratories, Stoney Road, Stormont, BT4 3SD Belfast, UK |
| |
| Abstract: | Ovine, bovine and porcine plasma glutathione peroxidase (GSH-Px) activity decreased on storage at both 4°C and 20°C. The ovine and bovine enzymes were significantly less stable than the porcine enzyme. The addition of GSH to a final concentration of 2 mmol/L to plasma samples at the commencement of storage retarded the loss of both ovine and bovine plasma GSH-Px activity. The ovine enzyme was unique in that after inactivation by storage at 4°C, incubation with GSH restored the enzyme activity. It is recommended that plasma GSH-Px should be assayed fresh, or otherwise stored at –20°C. |
| |
| Keywords: | cattle glutathione peroxidase plasma pig sheep stability storage |
| 本文献已被 SpringerLink 等数据库收录！ |
