| 伊犁地区绵羊李氏杆菌药敏特性及平板凝集快速诊断方法的建立 |
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| 引用本文： | 皮志媛,王一明,张海兰,雷程红.伊犁地区绵羊李氏杆菌药敏特性及平板凝集快速诊断方法的建立[J].安徽农业科学,2014(2):480-482. |
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| 作者姓名： | 皮志媛 王一明 张海兰 雷程红 |
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| 作者单位： | 新疆农业大学,新疆乌鲁木齐830052;伊犁职业技术学院,新疆伊犁835000;伊犁职业技术学院,新疆伊犁,835000;新疆农业大学,新疆乌鲁木齐,830052 |
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| 基金项目： | 新疆维吾尔自治区高校科研计划青年科研基金项目（xjedu2011s48）. |
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| 摘 要： | 目的]建立一种快速检测李氏杆菌病的方法。方法]通过对实验室保存的5株李氏杆菌分离株进行药敏试验,用甲醛灭活苗免疫试验动物,制备特异性抗体,将特异性抗体与抗原进行平板凝集试验。结果]分离菌株对氨苄西林、庆大霉素、链霉素高度敏感,对头孢唑啉、左氧氟沙星和多粘菌素中度敏感,对头孢他啶、头孢呋辛钠耐药性较强。通过用甲醛灭活苗免疫试验动物来制备特异性抗体,将特异性抗体与抗原做平板凝集试验,建立一种快速准确检测李氏杆菌病的方法。结论]成功建立了一种快速检测李氏杆菌病的方法。
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| 关 键 词： | 李氏杆菌 药敏特性 快速诊断方法 |
Drug Sensitivity Characteristics of Listeria monocytogenes and Establishment of Plate Agglutination Fast Diagnostic Technique in Yili Region |
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| Institution: | PI Zhi-yuan et al (Xinjiang Agricultural University, Yili, Xinjiang 830052) |
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| Abstract: | Objective ] The research aimed to establish a rapid determination method of listeriosis. Method ] Five strains of Listeria monocy- togenes preserved in the laboratory were use to make drug sensitivity test. The test animals were immunized by using formaldehyde-inactivated vaccine to make specific antibody. The specific antibody and antigen were used to make plate agglutination test. Result] The isolated strains were highly-sensitive to ampicillin, gentamicin and streptomycin, moderately- sensitive to cefazolin, levofloxacin and polymyxin. And the iso- lated strains were highly-toleratn to ceftazidime and cefuroxime sodium. The test animals were immunized by formaldehyde-inactivated vaccine to make specific antibody. The specific antibody and antigen was used to make plate agglutination test. Thus a rapid and accurate detection method of listeriosis was established. Conclusion] A rapid detection method of listeriosis was successfully established. |
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| Keywords: | Listeria monocytogenes Drug sensitivity characteristics Rapid diagnostic method |
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