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Evaluation of Germplasm Using SSR Markers of Functional Genes in Rice
Authors:ZHAO Yong  YANG Kai  Akbar Ali Cheema  WENG Yue-jin
Institution:1. Institute of Crop Germplasm Resources, Chinese Academy of Agricultural Sciences, Beijing 100081, P.R. China
2. Nuclear Institute for Agriculture and Biology, Faisalabad, P. O. Box 128, Pakistan
Abstract:16 SSR (Simple sequence repeats) primers of functional genes in rice were used to detect genetic diversity among 23 accessions of rice germplasm from 5 countries in the world. The average number of alleles per SSR locus was 5.2 with a range from 2 to 10. Genetic similarities among the 23 rice accessions ranged from 0.13 to 0.64. UPGMA cluster analysis showed that the 23 rice accessions could be classified into two distinct classes at similarities with a coefficient of 0.13. The Japonicas from Brazil, Japan and China were classified into Class I, along with upland rice from Brazil. The Indicas from Pakistan and Korea were classified into Class Ⅱ. Consequently, the function of genes SSR markers could be used as a useful tool for measuring genetic diversity, assigning rice to geographical distribution, ecotype, and pedigree relationship.
Keywords:Rice  SSR  Cluster analysis
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