| 橡胶延长因子基因的原核表达及其多克隆抗体的制备(英文) |
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| 引用本文： | 陈章权,吴坤鑫,梁晓东,黄震,陆田田.橡胶延长因子基因的原核表达及其多克隆抗体的制备(英文)[J].农业科学与技术,2008,9(4):29-33. |
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| 作者姓名： | 陈章权 吴坤鑫 梁晓东 黄震 陆田田 |
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| 作者单位： | 广东医学院临床免疫学教研室;中国热带农业科学院热带生物技术研究所 |
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| 基金项目： | 国家自然科学基金，中央级公益科研究院所基本科研业务费专项资金项目 |
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| 摘 要： | Objective] The aim of this study was to prepare the recombination protein of rubber elongation factor and its polyclonal antibodies.Method] The encoding gene of rubber elongation factor(REF)was amplified by RT-PCR,and cloned into the prokaryotic expression vector pDEST17 to transform into Escherichia coil BI2I-AI.The recombinant protein induced by L-Arabinose was purified by the affinity chromatography.As the immunogen,the recombination protein was used to immunize mice for preparing polyclonal antibodies,while ELISA and Western blot hybridization were used to detect the titers and specificity.Result] The purified recombination protein of REF with high expression was used to immunize house mice for preparing polyclonal antibodies with high titer and specificity.The western blot hybridization showed that the antibody could recognize the natural REF from latex.Conclusion] The recombination protein of REF was successfully obtained and the mouse anti REF antibody with high titer and specificity was prepared,which lays a basis for further studies on biological functions of rubber elongation factor and other membrane proteins in rubber particles.
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| 关 键 词： | Rubber elongation factor Gene Expression Antibody Preparation |
Prokaryotic Expression of Rubber Elongation Factor Gene and Preparation of Its Polyclonal Antibody |
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| CHEN Zhang-quan,WU Kun-xin,LIANG Xiao-dong,HUANG Zhen,LU Tian-tian.Prokaryotic Expression of Rubber Elongation Factor Gene and Preparation of Its Polyclonal Antibody[J].Agricultural Science ＆ Technology,2008,9(4):29-33. |
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| Authors: | CHEN Zhang-quan WU Kun-xin LIANG Xiao-dong HUANG Zhen LU Tian-tian |
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| Institution: | 1.Department of Clinical Immunology,Guangdong Medical College,Zhanjiang 524023;2.Institute of Tropical Bioscience and Biotechnology,Chinese Academy of Tropical Agricultural Science,Haikou 571101 |
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| Abstract: | Objective] The aim of this study was to prepare the recombination protein of rubber elongation factor and its polyclonal antibodies. Method] The encoding gene of rubber elongation factor (REF) was amplified by RT-PCR, and cloned into the prokaryotic expression vector pDEST17 to transform into Escherichia coil BI2I-AI. The recombinant protein induced by L-Arabinose was purified by the affinity chromatography. As the immunogen, the recombination protein was used to immunize mice for preparing polyclonal antibodies, while ELISA and Western blot hybridization were used to detect the titers and specificity. Result] The purified recombination protein of REF with high expression was used to immunize house mice for preparing polyclonal antibodies with high titer and specificity. The western blot hybridization showed that the antibody could recognize the natural REF from latex. Conclusion] The recombination protein of REF was successfully obtained and the mouse anti REF antibody with high titer and specificity was prepared, which lays a basis for further studies on biological functions of rubber elongation factor and other membrane proteins in rubber particles. |
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| Keywords: | Rubber elongation factor Gene Expression Antibody Preparation |
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