| 茅膏菜试管苗不同增殖方式研究 |
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| 引用本文： | 赖恭梯,刘海林,郭梽超,林玉玲,赖钟雄.茅膏菜试管苗不同增殖方式研究[J].甘蔗(福建),2011(1):57-63. |
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| 作者姓名： | 赖恭梯 刘海林 郭梽超 林玉玲 赖钟雄 |
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| 作者单位： | 福建农林大学园艺植物生物工程研究所,福建福州350002 |
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| 基金项目： | 国家级大学生创新性实验资助项目（091038904） |
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| 摘 要： | 为解决茅膏菜离体快繁中试管苗增殖的困难,本试验以茅膏菜试管苗为材料,通过3种不同增殖方式,研究不同激素组合、浓度的培养基对其增殖的影响。结果表明,①丛生芽增殖方式的最佳培养基为：1/2MS＋0.1 mg·L^-1 6-BA＋0.1 mg·L^-1 NAA,时间为55 d;②叶片愈伤组织诱导的最佳培养基为：1/2MS＋1.0 mg·L^-1 6-BA＋0.1 mg·L^-1NAA,时间为15-20 d,愈伤组织分化的最佳培养基为1/2MS,时间为15-20 d;③茅膏菜叶片直接诱导不定芽增殖方式的最佳培养基为1/2MS,时间为30 d。
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| 关 键 词： | 茅膏菜 增殖 愈伤组织 丛生芽 不定芽 |
Study on proliferation in different ways of Drosera spatulata |
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| LAI Gong-ti,LIU Hai-lin,GUO Zhi-chao,LIN Yu-ling,LAI Zhong-xiong.Study on proliferation in different ways of Drosera spatulata[J].Sugarcane,2011(1):57-63. |
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| Authors: | LAI Gong-ti LIU Hai-lin GUO Zhi-chao LIN Yu-ling LAI Zhong-xiong |
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| Institution: | (Institute of Horticultural Biotechnology,Fujian Agriculture and Forestry University,Fuzhou,Fujian 350002,China) |
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| Abstract: | In this experiment,to solve the difficulties of in vitro propagation of the proliferation,the sterile seedlings were used as experimental materials to study the effects of different hormone combinations and concentrations on the induction and proliferation through 3 different proliferation ways in sundew（Drosera spatulata）.The obtained results showed that： ① the best medium for the multiple bud cluster proliferation within 55 days was 1/2 MS＋0.1 mg·L^-1 6-BA＋0.1 mg·L^-1 NAA;② the best induction medium for the formation of callus from leaves within 20 days was 1/2 MS＋1.0 mg·L^-1＋0.1 mg·L^-1 NAA,and the best differentiation medium for callus within 20 days was 1/2 MS;③ the best medium for the adventitious buds formation directly from the leaves within 30 days was 1/2 MS. |
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| Keywords: | sundew（Drosera spatulata） proliferation callus multiple bud cluster adventitious bud |
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