| 小麦TaUCH基因的克隆和特性分析 |
| |
| 引用本文： | 秘彩莉.小麦TaUCH基因的克隆和特性分析[J].麦类作物学报,2010,30(6). |
| |
| 作者姓名： | 秘彩莉 |
| |
| 作者单位： | 河北师范大学生命科学学院 |
| |
| 基金项目： | 国家自然科学基金（30370881, 30771348）和河北师范大学博士基金(L2005B22) |
| |
| 摘 要： | 摘要：在以前的工作中，本课题组通过cDNA-AFLP得到了一个219bp的泛素羧基末端水解酶的片段，本文以该片段为基础，通过电子延伸、RACE和RT-PCR，得到了两个全长cDNA克隆，分别命名为TaUCH1和TaUCH2，这两条序列在编码区只有一个氨基酸的差异。保守结构域预测显示，TaUCH1和TaUCH2的中部和C末端各有一个C19肽酶结构域。将TaUCH及其在植物中同源蛋白进行对比发现，TaUCH与水稻中的同类蛋白相似性最高。Southern杂交显示，TaUCH在小麦中以多拷贝或基因家族形式存在。
|
| 关 键 词： | 小麦 TaUCH 克隆 特性分析 |
| 收稿时间： | 8/25/2010 4:32:26 PM |
Cloning and characterization of TaUCH in Wheat |
| |
| BI Cai-li.Cloning and characterization of TaUCH in Wheat[J].Journal of Triticeae Crops,2010,30(6). |
| |
| Authors: | BI Cai-li |
| |
| Abstract: | Abstract: In previous work, a 219bp cDNA fragment sharing high similarity with ubiquitin C-terminal hydrolase was obtained by cDNA-AFLP, based on this fragment, two full length cDNAs were cloned via silico cloning, RACE and RT-PCR, and named as TaUCH1 and TaUCH2 respectively. They are almost identity except for an amino acid diferrence in the open reading frame. Conserved domain prediction indicated that there are C19 peptidase domains both in the middle and at the C-terminal of the two proteins. Phylogenetic analysis indicated that TaUCH1 and TaUCH2 share the highest similarity with their homologous proteins in rice. Southern blot revealed that TaUCHs consist of multiple copies or a gene family in wheat. |
| |
| Keywords: | wheat TaUCH cloning characterization |
|
| 点击此处可从《麦类作物学报》浏览原始摘要信息 |
| 点击此处可从《麦类作物学报》下载免费的PDF全文 |
