| 甘蔗单花粉全基因组扩增(WGA)与SCoT分子标记研究 |
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| 引用本文： | 陈平华,陈如凯,许莉萍,王恒波,陈利平,林冰,施桂姣,张卓,高三基,郭晋隆,潘永保.甘蔗单花粉全基因组扩增(WGA)与SCoT分子标记研究[J].热带作物学报,2011,32(11):2069-2075. |
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| 作者姓名： | 陈平华 陈如凯 许莉萍 王恒波 陈利平 林冰 施桂姣 张卓 高三基 郭晋隆 潘永保 |
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| 作者单位： | 福建农林大学农业部甘蔗遗传改良重点开放实验室;福建农林大学农业部甘蔗遗传改良重点开放实验室;福建农林大学农业部甘蔗遗传改良重点开放实验室;福建农林大学农业部甘蔗遗传改良重点开放实验室;福建农林大学农业部甘蔗遗传改良重点开放实验室;福建农林大学农业部甘蔗遗传改良重点开放实验室;福建农林大学农业部甘蔗遗传改良重点开放实验室;福建农林大学农业部甘蔗遗传改良重点开放实验室;福建农林大学农业部甘蔗遗传改良重点开放实验室;福建农林大学农业部甘蔗遗传改良重点开放实验室;福建农林大学农业部甘蔗遗传改良重点开放实验室 |
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| 基金项目： | 国家基金项目（No. 31070330），国家948项目（No. 2010-S19），福建省科技计划重点项目（No. 2007I0036）。 |
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| 摘 要： | 从一个完整的甘蔗花粉囊中分离出单个花粉粒,利用特制取样器进行收集,采用碱裂解法释放单花粉DNA,裂解液可以直接作为WGA模板进行全基因组扩增,制备出单个花粉粒大量DNA.通过5S rRNA-ITS鉴定WGA产物真实性,并以目标起始密码子多态性标记对真实的WGA产物进行分析,显示出丰富的多态性.利用NTSYSpc软件分析...
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| 关 键 词： | 甘蔗 单花粉 全基因组扩增 SCoT分子标记 遗传分布 |
Whole Genome Amplification of Single Pollen Grains from a Sugarcane Cultivar and Analysis of the Genetic Relatedness based on SCoT Markers |
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| CHEN Pinghu,CHEN Rukai,XU Liping,WANG Hengbo,CHEN Liping,LIN Bing,SHI Guijiao,ZHANG Zhuo,GAO Sanji,GUO Jinlong and PAN Yongbao.Whole Genome Amplification of Single Pollen Grains from a Sugarcane Cultivar and Analysis of the Genetic Relatedness based on SCoT Markers[J].Chinese Journal of Tropical Crops,2011,32(11):2069-2075. |
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| Authors: | CHEN Pinghu CHEN Rukai XU Liping WANG Hengbo CHEN Liping LIN Bing SHI Guijiao ZHANG Zhuo GAO Sanji GUO Jinlong and PAN Yongbao |
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| Institution: | Key Lab of Sugarcane Genetic Improvement, Ministry of Agriculture, Fujian Agriculture and Forestry University,;Key Lab of Sugarcane Genetic Improvement, Ministry of Agriculture, Fujian Agriculture and Forestry University,;Key Lab of Sugarcane Genetic Improvement, Ministry of Agriculture, Fujian Agriculture and Forestry University,;Key Lab of Sugarcane Genetic Improvement, Ministry of Agriculture, Fujian Agriculture and Forestry University,;Key Lab of Sugarcane Genetic Improvement, Ministry of Agriculture, Fujian Agriculture and Forestry University,;Key Lab of Sugarcane Genetic Improvement, Ministry of Agriculture, Fujian Agriculture and Forestry University,;Key Lab of Sugarcane Genetic Improvement, Ministry of Agriculture, Fujian Agriculture and Forestry University,;Key Lab of Sugarcane Genetic Improvement, Ministry of Agriculture, Fujian Agriculture and Forestry University,;Key Lab of Sugarcane Genetic Improvement, Ministry of Agriculture, Fujian Agriculture and Forestry University,;Key Lab of Sugarcane Genetic Improvement, Ministry of Agriculture, Fujian Agriculture and Forestry University,;Key Lab of Sugarcane Genetic Improvement, Ministry of Agriculture, Fujian Agriculture and Forestry University, |
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| Abstract: | |
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| Keywords: | Sugarcane Single pollen grain Whole cenome amplification SCoT markers Genetic relatedness |
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