| 花生查尔酮异构酶基因的克隆及其序列分析 |
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| 引用本文： | 温世杰,邱金梅,刘海燕,朱方何,王文皓,梁炫强.花生查尔酮异构酶基因的克隆及其序列分析[J].热带作物学报,2011,32(11):2084-2087. |
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| 作者姓名： | 温世杰 邱金梅 刘海燕 朱方何 王文皓 梁炫强 |
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| 作者单位： | 广东省农业科学院作物研究所;华南师范大学生命科学学院;广东省农业科学院作物研究所;广东省农业科学院作物研究所;华南师范大学生命科学学院;1 广东省农业科学院作物研究所
2 华南师范大学生命科学学院 |
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| 基金项目： | 现代农业产业技术体系建设专项资金（No. nycyta-19）；粤港关键领域重点突破项目（No. 200849863）。 |
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| 摘 要： | 利用RT-PCR及RACE技术从花生种子中克隆得到查尔酮异构酶基因(chi)的cDNA全长序列,命名为Ahchi,NCBI登录号:JN412735,该序列全长共1 061 bp,编码209个氨基酸,其氨基酸组成与其它已知的高等植物具有很高的同源性,与大豆、倒捻子、蓖麻、毛果杨的同源性分别为81%、71%、70%和69%,且含有高度保守的活性位点。
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| 关 键 词： | 花生 查尔酮异构酶基因( ) 基因克隆 |
Cloning and Sequence Analysis of cDNA of Chalcone Isomerase Gene from Peanut(Aras hypogaea L.) |
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| WEN Shijie,QIU Jinmei,LIU Haiyan,ZHU Fanghe,WANG Wenhao and LIANG Xianqiang.Cloning and Sequence Analysis of cDNA of Chalcone Isomerase Gene from Peanut(Aras hypogaea L.)[J].Chinese Journal of Tropical Crops,2011,32(11):2084-2087. |
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| Authors: | WEN Shijie QIU Jinmei LIU Haiyan ZHU Fanghe WANG Wenhao and LIANG Xianqiang |
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| Institution: | Crops Research Institute, Guangdong Academy of Agricultural Sciences;College of Life Sciences, South na Normal University;Crops Research Institute, Guangdong Academy of Agricultural Sciences;Crops Research Institute, Guangdong Academy of Agricultural Sciences;College of Life Sciences, South na Normal University;1 Crops Research Institute, Guangdong Academy of Agricultural Sciences 2 College of Life Sciences, South na Normal University |
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| Abstract: | The cDNA sequence of the chalcone isomerase gene(Ah)numbered JN412735 in GeneBank was amplified by RT-PCR and RACE from Aras hypogaea L.. The anlyasis indicated that the cDNA sequence was 106 1 bp, encoded 209 amino acids with high homology to from other higher plants, contained the highly conserved catalytic active sites . |
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| Keywords: | Peanut(Aras hypogaea L ) Gene clone |
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