| 文昌鸡PRL基因的克隆及序列分析 |
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| 引用本文： | 周海龙,林 丛,韦双双,廖承红,江 明,何大乾.文昌鸡PRL基因的克隆及序列分析[J].热带作物学报,2011,31(2):39-43. |
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| 作者姓名： | 周海龙 林 丛 韦双双 廖承红 江 明 何大乾 |
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| 作者单位： | 海南大学农学院 海南海口 570228;海南大学农学院 海南海口 570228;海南大学农学院 海南海口 570228;海南大学农学院 海南海口 570228;海南大学农学院 海南海口 570228;上海市农业科学院畜牧兽医研究所 上海 201106 |
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| 摘 要： | 为研究文昌鸡催乳素基因的分子生物学特性和就巢机理，提取文昌鸡全血总DNA，利用特异性引物PCR技术获得文昌鸡PRL基因，将其克隆至pGM-T载体上，并测序分析。结果表明，文昌鸡PRL基因与GenBank上已公布的白来航、泰和丝羽乌骨鸡核苷酸序列同源性分别为93.3%和93.5%，而氨基酸序列同源性均为89.6%；与其他动物PRL基因进行比较，发现亲缘关系越远，同源性越低。
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| 关 键 词： | 文昌鸡 催乳素基因 克隆 序列分析 |
Cloning and Sequence Analysis of Prolactin Gene from Wenchang Chicken |
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| Institution: | Agricultural College, Hainan University, Haikou, Hainan 570228;Agricultural College, Hainan University, Haikou, Hainan 570228;Agricultural College, Hainan University, Haikou, Hainan 570228;Agricultural College, Hainan University, Haikou, Hainan 570228;Agricultural College, Hainan University, Haikou, Hainan 570228;Institute of Animal Husbandry & Veterinary Science, Shanghai Academy of Agricultural Science, Shanghai 201106 |
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| Abstract: | The molecular biological characteristics and hatching behavior mechanism of Wenchang chicken were studied. Total DNA was extracted from whole blood of Wenchang chicken. The Wenchang chicken prolactin gene was amplified using PCR with specific primer, cloned to pGM-T and sequenced. The results showed that the nucleotide homologies between Wenchang chicken gene and White leghorn, Taihe silkies prolactin genes were 93.3% and 93.5% respectively, the amino acid homologies of them were 89.6%. The nucleotide and amino acid homologies of Wenchang chicken with Turkey, Indian peafowl, Ostrich, Human, etc. decreased in accordance with their far genetic relationships. |
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| Keywords: | Wenchang chicken prolactin gene cloning sequence analysis |
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