| 基于AFLP的海萝野生群体遗传多样性分析 |
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| 引用本文： | 郭奕惠,范嗣刚,陈素文,喻达辉,吴进锋,陈利雄,朱长波,郭永坚.基于AFLP的海萝野生群体遗传多样性分析[J].中国水产科学,2015,22(1):158-163. |
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| 作者姓名： | 郭奕惠 范嗣刚 陈素文 喻达辉 吴进锋 陈利雄 朱长波 郭永坚 |
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| 作者单位： | 农业部南海渔业资源开发利用重点实验室,中国水产科学研究院 南海水产种质资源与健康养殖重点实验室,南海水产研究所,广东 广州 510300 |
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| 基金项目： | 广东省海洋渔业科技推广专项,中央级公益性科研院所基本科研业务费,农业部东海海水健康养殖重点实验室 |
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| 摘 要： | 应用AFLP分子标记技术对广东深圳、汕头和山东长岛的海萝(Gloiopeltis furcata)群体进行遗传多样性分析。应用筛选出的10对多态性丰富的AFLP引物,对这3个地理群体的90个个体进行扩增,共得到427个位点,多态性位点数为392(91.75%),各引物扩增位点数为30~59。长岛群体扩增位点最多,多态性也最高。群体内的等位基因数(Na)、有效等位基因数(Ne)、遗传多样性指数(H)和Shannon信息指数(I)变化趋势一致,均由高到低依次为长岛群体、汕头群体、深圳群体,表明长岛群体遗传多样性最高。3个海萝群体遗传变异主要来自群体间。群体间的基因流为0.368 9,群体分化系数(Gst)为0.575 4,表明群体间有高度分化。深圳群体和汕头群体的遗传距离最小(0.110 2),与长岛群体的遗传距离最大(0.357 7)。UPGMA聚类分析显示广东深圳和汕头群体聚为一支,山东长岛群体为另一支,表明群体间的遗传差异与地理距离有关。本研究结果为海萝资源的保护与利用提供了基础数据。
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| 关 键 词： | AFLP 海萝 遗传多样性 地理群体 |
| 修稿时间： | 2015/6/17 0:00:00 |
Analysis of genetic diversity in wild populations of Gloiopeltis furcata based on amplified fragment length polymorphism methodology |
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| GUO Yihui,FAN Sigang,CHEN Suwen,YU Dahui,WU Jinfeng,CHEN Lixiong,ZHU Changbo,GUO Yongjian.Analysis of genetic diversity in wild populations of Gloiopeltis furcata based on amplified fragment length polymorphism methodology[J].Journal of Fishery Sciences of China,2015,22(1):158-163. |
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| Authors: | GUO Yihui FAN Sigang CHEN Suwen YU Dahui WU Jinfeng CHEN Lixiong ZHU Changbo GUO Yongjian |
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| Institution: | Key Laboratory of South China Sea Fishery Resources Exploitation & Utilization, Ministry of Agriculture; Key La-borat¬ory of Fisheries Genetic Resources and Aquaculture, South China Sea Fisheries Research Institute, Chinese Academy of Fisheries Scienc |
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| Abstract: | is a perennial red alga (Rhodophyta) commonly used as a source of food, drugs, or textile industry raw materials. Recently, has been used for artificial breeding experiments and aquaculture research. However, limited genetic information about this species has hindered progress in artificial culture of it. In this study, we estimated genetic diversity in using amplified fragment length polymorphism markers with three populations of 90 individuals from Shenzhen, Shantou, and Changdao, China. Ten pairs of polymorphic primers were screened, generating 427 loci, of which 392 (91.75 %) were polymorphic. Loci obtained from each pair of primers ranged from 30 to 59. The Changdao population possessed the highest number of amplified loci and the highest polymorphism level. The observed number of alleles () showed the same trend in the three populations (i.e., Changdao>Shantou>Shenzhen), suggesting that the Changdao population had the highest genetic diversity. Major genetic variation existed among the populations. was 0.575 4, indicating high genetic differentiation among the populations. The smallest genetic distance was found (0.110 2), while the largest was between the Shenzhen and Changdao populations (0.357 7). The Shenzhen and Shantou populations clustered together and then grouped with the Changdao population, suggesting that the genetic divergence observed was related to geographical distance. Our results resources. |
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| Keywords: | amplified fragment length polymorphism (AFLP) Gloiopeltis furcata genetic diversity geographical populations |
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