| 酶联免疫吸附试验检测禽脑脊髓炎抗体方法的研究 |
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| 引用本文： | 秦卓明,赵继勋,杨建民,金维江,张世栋,贾强.酶联免疫吸附试验检测禽脑脊髓炎抗体方法的研究[J].山东农业科学,2002(1):13-15,39. |
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| 作者姓名： | 秦卓明 赵继勋 杨建民 金维江 张世栋 贾强 |
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| 作者单位： | 1. 山东省农业科学院家禽研究所SPF鸡场,山东,济南,250100
2. 中国农业大学,北京,100094 |
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| 摘 要： | 利用鸡胚成纤维细胞 (CEF)繁育禽脑脊髓炎病毒 (AEV) ,取其培养物 ,经差速离心提纯 ,做为酶联包被杭原 ,建立了检测AEV抗体的间接酶联免疫吸附试验 ,初步确定了各种反应条件 :抗原包被浓度 16 7μg/ml,待检血清 1∶10 0稀释 ,酶标抗体工作浓度 1∶5 0 0。以阴性血清OD值平均值加上 2个标准差作为界值 ,当待测样品OD值大于界值 0 2 7时 ,判为阳性 ,否则判为阴性。经交叉试验、阻断试验、重复试验和平行试验 ,证实了本试验建立的方法具有良好的特异性和重复性
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| 关 键 词： | 禽脑脊髓炎 鸡胚成纤维细胞 酶联免疫吸附试验 |
| 文章编号： | 1001-4942(2002)01-0013-03 |
Study on ELISA method to detect the antibody of AEV |
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| Abstract: | The ELISA method to detect the antibody of AEV was established,which purified the AEV bred by CEF and used it as enveloped antigen Conditions of reaction were as follows: consistence of enveloped antigen was 16 7μg/ml, the unknown serum was dilted by 1∶100, consistence of enzyme-linked antibody activated in 1∶500 Using average OD of negative serum plus the two standard deviations as critical value,it was judged positive when the OD of the unknown serum higher than 0 27, otherwise it was negative It was proved that the method was well special and repeatable by cross experiment, interdiction experiment, repeated experiment and parallel experiment |
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| Keywords: | Avian Encephalomylitis CEF ELISA |
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