| 水稻遗传转化甘露糖安全筛选体系的建立 |
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| 引用本文： | 王彩芬,付永彩,朱作峰,徐杰,葛占宇,孙传清.水稻遗传转化甘露糖安全筛选体系的建立[J].西北农业学报,2007,16(2):41-45. |
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| 作者姓名： | 王彩芬 付永彩 朱作峰 徐杰 葛占宇 孙传清 |
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| 作者单位： | 1. 中国农业大学植物遗传育种系,农业生物技术国家重点实验室,教育部作物杂种优势研究与利用重点实验室,农业部农作物基因组学与遗传改良重点开放实验室,北京市作物遗传改良重点实验室,北京,100094;宁夏农林科学院农作物研究所,宁夏永宁,750105
2. 中国农业大学植物遗传育种系,农业生物技术国家重点实验室,教育部作物杂种优势研究与利用重点实验室,农业部农作物基因组学与遗传改良重点开放实验室,北京市作物遗传改良重点实验室,北京,100094 |
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| 摘 要： | 根据genebank中公布的pmi基因序列设计引物,通过PCR扩增从大肠杆菌中分离出6-磷酸甘露糖异构酶基因。将该基因替换植物表达载体pCAMBIA1300中的潮霉素抗性基因hpt,构建了以pmi为选择标记基因的植物表达载体pPMI。对水稻进行了基因枪转化和农杆菌转化,研究了筛选培养基中甘露糖浓度对抗性愈伤组织频率的影响。部分抗性愈伤组织已获得再生植株,PCR检测初步证明外源基因已转入植物细胞。
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| 关 键 词： | 6-磷酸甘露糖异构酶基因(pmi) 甘露糖 安全筛选标记 水稻 遗传转化 |
| 文章编号： | 1004-1389（2007）02-0041-05 |
| 收稿时间： | 2006-11-20 |
| 修稿时间： | 2006-12-10 |
A Security Selection System for Rice Transformation by Mannose |
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| WANG Cai-fen,FU Yong-cai,ZHU Zuo-feng,XU Jie,GE Zhan-yu and SUN Chuan-qing.A Security Selection System for Rice Transformation by Mannose[J].Acta Agriculturae Boreali-occidentalis Sinica,2007,16(2):41-45. |
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| Authors: | WANG Cai-fen FU Yong-cai ZHU Zuo-feng XU Jie GE Zhan-yu and SUN Chuan-qing |
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| Institution: | 1. Department of Plant Genetic and Breeding, China Agricultural University,State Key Laboratory for Agrobiotechnology, Key Laboratory of Crop Heterosis and Utilization of Ministry of Education,Key Laboratory of Crop Improvement and Genome of Ministry of Agriculture, Beijing 100094,China;2. Crop Institute of Ningxia Agriculture and Forestry Academy, Yongning Ningxia 750105,China |
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| Abstract: | According to pmi gene sequence in genebank,the 6-phosphomarmose isomerase gene of Escherichia coli was amplified by PCR.Based on pCAMBIA1300,the plant expression vector with pmi was constructed by substituting pmi for hygromycin resistance gene.Rice transformation was conducted by Microprojectile bombardment and by Agrobacterium mediated pathway. Research had been done on the percentage of resistant calli influenced by mannose concentration in selection medium.Some of the transgenic plants had been obtained.Transformation was confirmed by PCR. |
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| Keywords: | 6-Phosphomarmose isomerase gene Mannose Security selection marker Rice Transformation |
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