Expression of Frizzled 2 in the mouse ovary during oestrous cycle |
| |
| Authors: | S B Wang B S Xing L Yi W Wang Y X Xu |
| |
| Institution: | 1. College of Animal Science and Technology, Nanjing Agricultural University, Nanjing, China;2. Animal Husbandry and Veterinary Institute, Henan Academy of Agricultural Sciences, Zhenzhou, China;3. Taicang Guangdong Wen’s poultry Ltd, Taicang, China;4. Taicang Guangdong Wen’s poultry Ltd, Taicang, China |
| |
| Abstract: | The Wnt/β‐catenin pathway is a conserved signalling pathway that regulates gene expression and controls diverse developmental processes such as cell fate specification, cell proliferation and cell differentiation. To our knowledge, the potential role of this pathway in the adult ovary has been poorly addressed. To this end, we investigated the expression pattern of Frizzled 2 in the mouse ovary during oestrous cycle by real‐time Q‐PCR, in situ hybridization, Western blotting and immunohistochemistry. In this study, we used uterine wet weight and H3.2 mRNA level as two markers for classification of mouse oestrous cycle. We found that both uterine wet weight and H3.2 mRNA are the highest in the proestrus and decrease from oestrus to diestrus. During oestrous cycle, Frizzled 2 mRNA and protein exhibited the highest level in the proestrus stage and rapidly reduced from oestrus to diestrus. In situ hybridization results showed that the positive signals for Frizzled 2 are highly detected in the oocyte and stroma in proestrus stage, while moderate or weak Frizzled 2 mRNAs are localized in the oocyte, granulosa cells, stroma and corpus luteum from oestrus to diestrus. The localization pattern of Frizzled 2 protein is mostly consistent with its mRNA, but stronger Frizzled 2 proteins are present in the granulosa cells and membrane of oocyte in oestrous cycle. Our data suggest that Frizzled 2 may be involved in regulating follicle growth, oocyte maturation and luteinization during oestrous cycle. |
| |
| Keywords: | Frizzled 2 ovary oestrous cycle real‐time Q‐PCR in situ hybridization Western blotting immunohistochemistry |
|
|
