| 蓝塘猪和长白猪leptin和leptin受体基因表达的变化 |
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| 引用本文： | 陈赞谋,戚晓鸿,张 豪,吴珍芳,刘德武.蓝塘猪和长白猪leptin和leptin受体基因表达的变化[J].华中农业大学学报,2010,29(1):67-70. |
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| 作者姓名： | 陈赞谋 戚晓鸿 张 豪 吴珍芳 刘德武 |
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| 作者单位： | 华南农业大学动物科学学院,广州,510642 |
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| 基金项目： | 国家自然科学基金项目(30571327);;广东省农业攻关项目(2008A020100001和2007B020706004)资助 |
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| 摘 要： | 用实时荧光定量PCR方法研究了蓝塘猪和长白猪1、27、90、150和180日龄阶段脂肪组织中leptin基因mRNA和下丘脑中leptin受体基因mRNA的变化,并对2个品种不同生长阶段血液中leptin含量的变化规律进行了探讨。结果表明:(1)蓝塘猪180日龄时皮下脂肪中leptin基因表达量显著高于其它各阶段,而与长白猪相比,蓝塘猪除1日龄低于长白猪外,其它各个阶段均高于长白猪,180日龄两者差异极显著;长白猪各阶段间差异不显著;(2)蓝塘猪180日龄时下丘脑中leptin受体基因的表达量显著高于其它各阶段,长白猪180日龄时最高,且显著高于1日龄和27日龄,蓝塘猪与长白猪1日龄时差异显著;(3)蓝塘猪血清中leptin浓度各阶段均高于长白猪,两品种均是150日龄时最高,蓝塘猪150日龄显著高于27日龄和180日龄,长白猪各阶段差异不显著。
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| 关 键 词： | 猪 leptin leptin受体 基因表达 |
| 收稿时间： | 2008/6/28 0:00:00 |
| 修稿时间： | 2008/12/30 0:00:00 |
Changes of Leptin and Leptin Receptor Gene Expression in Subcutaneous Fat and Hypothalamus of Lantang and Landrace Pigs |
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| CHEN Zan-mou,QI Xiao-hong,ZHANG Hao,WU Zhen-fang and LIU De-wu.Changes of Leptin and Leptin Receptor Gene Expression in Subcutaneous Fat and Hypothalamus of Lantang and Landrace Pigs[J].Journal of Huazhong Agricultural University,2010,29(1):67-70. |
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| Authors: | CHEN Zan-mou QI Xiao-hong ZHANG Hao WU Zhen-fang and LIU De-wu |
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| Institution: | CHEN Zan-mou QI Xiao-hong ZHANG Hao WU Zhen-fang LIU De-wu College of Animal Science,South China Agricultural University,Guangzhou 510642,China |
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| Abstract: | Real time RT-PCR was applied to study the developmental patterns of leptin and leptin receptor gene mRNA expression at the ages of 1,27,90,150 and 180 days after born(DAB) in subcutaneous fat and hypothalamus of Lantang and Landrace pigs.The results showed that:(1) in subcutaneous fat,Lantang had the highest leptin mRNA level at 180 DAB.Leptin mRNA at all ages in lantang was higher than that in landrace,except at 1 DAB.Leptin mRNA was significantly higher in Lantang pig than that in landrace pig at 180 DAB.... |
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| Keywords: | pig leptin leptin receptor gene expression |
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