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Enzyme activities in some types of peripheral leukocytes of thoroughbred race horses before and after the races
Authors:Hosoya M  Inoue A  Kimura N  Arai T
Institution:Division of Veterinary Biochemistry, Department of Veterinary Science, Nippon Veterinary and Animal Science University, 1-7-1 Kyonancho, Musashino, Tokyo 180-8602, Japan.
Abstract:Glucose, free fatty acid (FFA), triglyceride (TG) and immunoreactive insulin (IRI) concentrations in plasma and activities of enzymes related to energy metabolism in some types of peripheral leukocytes were measured in thoroughbred race horses before and after racing. Glucose, FFA, TG and IRI concentrations and enzyme activities did not change significantly in plasma. However, the activities of cytosolic hexokinase, malate dehydrogenase (MDH), mitochondrial MDH and aspartate aminotransferase decreased significantly in leukocytes of the horses after the races. The cytosolic ratio of MDH/LDH activity (ML ratio) in leukocytes decreased significantly after racing, and the ratio may be a useful indicator to evaluate energy metabolism in race horses.
Keywords:Leukocytes  Malate dehydrogenase  Race horse
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