| 稻属叶绿体DNA限制性片段长度多态性 |
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| 引用本文： | 朱世华,王明全.稻属叶绿体DNA限制性片段长度多态性[J].中国水稻科学,1999,13(3):139-142. |
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| 作者姓名： | 朱世华 王明全 |
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| 作者单位： | [1]宁波大学师范学院 [2]武汉大学生命科学学院 |
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| 基金项目： | 国家自然科学基金!38970440 |
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| 摘 要： | 对稻属（Oryza）的14个种和李氏禾属（Leersia）的 2个种共 138份材料进行了叶绿体 DNA限制性片段长度多态性的分析。用内切酶EcoRⅠ酶切、4 个叶绿体 DNA探针杂交，共发现10种叶绿体 DNA变异类型。在稻属各个种内没有观察到叶绿体 DNA的变异，叶绿体 DNA变异类型基本上以稻属的染色体组型水平划分，因此认为稻属的叶绿体 DNA在进化过程中变异程度很低。推测 CCDD组的O. alta、O. grandiglumis和O. latifolia 及 CC组的 O.officinalis 和 O. eichingeri各有共同的原始祖先。遗传距离分析显示 CC、CCDD、BBCC组之间亲缘关系很近，这3个组与 EE组亲缘关系也较近。李氏禾属的 2个种 L. perrieri 和 L. tisseranti 与稻属的遗传距离很远。
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| 关 键 词： | 稻属 李氏禾属 叶绿体 DNA 限制性片段长度多态性 遗传距离 |
| 收稿时间： | 1900-01-01; |
Chloroplast DNA Restriction Fragment Length Polymorphism in the Genus Oryza |
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| ZHU Shi-hua,WANG Ming-quan,WANG Xiang-ming.Chloroplast DNA Restriction Fragment Length Polymorphism in the Genus Oryza[J].Chinese Journal of Rice Science,1999,13(3):139-142. |
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| Authors: | ZHU Shi-hua WANG Ming-quan WANG Xiang-ming |
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| Institution: | ZHU Shi-hua1,WANG Ming-quan2,WANG Xiang-ming2 |
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| Abstract: | one hundred and thirty eight accessions of l4 species in the genus Oryza with two Leersia species were assayed for restriction fragment length polymorphism (RFLP) of the chloroplast DNA (cpDNA) using EcoR I and four probes of chloroplast DNA. Ten types of chloroplast DNA variation were observed. No polymophisms were detected within any speciesinvestigated. The classification of chloroplast genome types is in accordance with that of nuclear genomes of Oryza and it is inferred that variability of chloroplast DNA in genus Oryza is low. our data suggested that CCDD tetroploid species (o. alta,o.grandiglumis and o. latifolia ) and CC diploid species (o.of ficinalis and o. eichingeri ) with each other have a common ancestor while phylogenetic analysis indicated that CC,BBCC and CCDD genomes were closely related to one another andthey were rather closely related to EE genome a1so. Perrieri and tisseranti of genus Leersia have more far genetic distance fromgenus Oryza. |
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| Keywords: | Oryza Leersia chloroplast DNA restriction fragment length polymorphism (RFLP) genetic distance |
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