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| 利用SRAP标记研究紫花苜蓿和黄花苜蓿种质资源遗传多样性 | |
| 引用本文： | 张宇,于林清,慈忠玲.利用SRAP标记研究紫花苜蓿和黄花苜蓿种质资源遗传多样性[J].中国草地学报,2012,34(1):72-76. |
| 作者姓名： | 张宇 于林清 慈忠玲 |
| 作者单位： | 1. 内蒙古农业大学生态环境学院,内蒙古 呼和浩特,010019 2. 中国农业科学院草原研究所/农业部草原资源与生态重点开放实验室,内蒙古呼和浩特,010010 3. 内蒙古农业大学农学院,内蒙古 呼和浩特,010019 |
| 基金项目： | 国家自然科学基金项目“杂花苜蓿杂种优势及其遗传标记分析”(30671487);中央级公益性科研院所基本科研业务费专项资金(中国农业科学院草原研究所);“耐旱紫花苜蓿新品种中草3号中试与示范”(农业科技成果转化项目2011GB2326001) |
| 摘 要： | 利用SRAP分子标记对31份苜蓿材料遗传多样性进行研究,探究不同地域来源的苜蓿种质的遗传变异和亲缘关系。筛选出24对引物,经PCR扩增后获得320条片段,其中多态性片段有287条,每对引物组合均能扩增出7～17多态性条带,平均为11.96条多态性条带;采用类平均法(UPGMA)Nei氏遗传距离进行聚类,将31份苜蓿材料聚成5类,但个别同一地域来源的苜蓿品种被归到不同的类型中,表明试供苜蓿材料具有较高的异质性。 |
| 关 键 词： | SRAP 紫花苜蓿 黄花苜蓿 遗传多样性 |
Genetic Diversity of Medicago sativaand M.falcata Germplasm Resources Based on SRAP Markers |
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| ZHANG Yu , YU Lin-qing , CI Zhong-ling.Genetic Diversity of Medicago sativaand M.falcata Germplasm Resources Based on SRAP Markers[J].Chinese Journal of Grassland,2012,34(1):72-76. | |
| Authors: | ZHANG Yu YU Lin-qing CI Zhong-ling |
| Institution: | 1.College of Ecology and Environmental Science,Inner Mongolia Agricultural University, Hohhot 010019,China;2.Grassland Research Institute,Chinese Academy of Agricultural Sciences/Key Laboratory of Grassland Resources and Ecology,Hohhot 010010,China; 3.Agronomy College,Inner Mongolia Agricultural University,Hohhot 010019,China) |
| Abstract: | Genetic diversity of 31 accessions of Medicago sativa and M.falcata was evaluated by sequence-related amplified polymorphism(SRAP) markers in order to explore the genetic variation and relationship among alfalfa germplasm resources from different regions.320 fragments were amplified by 24 SRAP primers,among which 287 were polymorphic.The number of polymorphic fragments ranged from 7 to 17 bands with an average of 11.96 bands per primer combination.The tested materials could be divided into 5 groups by UPGMA cluster analysis,but the individuals which were in one population can not be clustered in a group entirely,indicating that there was a definite difference among alfalfa. |
| Keywords: | SRAP Medicago sativa M falcata Genetic diversity |
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