| 鸡粪除臭菌的分离筛选及除臭效果分析 |
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| 引用本文： | 李玥,李成成,李静,张潇月,张紫薇,黄议漫,刘浩,罗鸿,张小平,赵珂.鸡粪除臭菌的分离筛选及除臭效果分析[J].农业环境科学学报,2020,39(5):1103-1110. |
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| 作者姓名： | 李玥 李成成 李静 张潇月 张紫薇 黄议漫 刘浩 罗鸿 张小平 赵珂 |
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| 作者单位： | 四川农业大学资源学院,成都 611130;中国农业大学资源与环境学院,北京 100094;旺苍县农业农村局,四川 广元628200;泸州市农业农村局现代农业发展促进中心,四川 泸州 646000 |
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| 摘 要： | 以鸡粪堆肥为材料,筛选高效除臭菌株并构建复合菌系,为鸡粪的无害化处理提供具有除臭功能的菌种资源。首先,采用驯化富集法、平板稀释法、产气试验、除氨试验、除硫化氢试验以及纤维素降解试验筛选出鸡粪堆肥中的除臭菌;其次,通过16S rRNA基因测序鉴定菌株;最后,通过拮抗试验构建除臭菌系。研究分离得到5株具有除臭功能的菌株,分属于芽孢杆菌属(MS03,Bacillus sp.)、贝莱斯芽孢杆菌(MS07,Bacillus velezensis)、耐寒短杆菌(MS11,Brevibacterium frigoritolerans)、木糖葡萄球菌(MS42,Staphylococcus xylosus)和变异棒杆菌(MS82,Corynebacterium variabile)。复合菌系C2(MS03+MS07)和C4(MS03+MS82)的综合除臭率均高于60%,且明显高于单株菌株及其他复合菌系。研究表明,这两个由不同除臭菌组合而成的复合菌系在无害化处理鸡粪方面具有较大的潜力和应用前景。
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| 关 键 词： | 鸡粪 除臭微生物 分离筛选 除臭效果 复合菌系 |
| 收稿时间： | 2019/12/9 0:00:00 |
Isolation and screening of deodorant bacterial strains from chicken manure and analysis of their deodorant effects |
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| LI Yue,LI Cheng-cheng,LI Jing,ZHANG Xiao-yue,ZHANG Zi-wei,HUANG Yi-man,LIU Hao,LUO Hong,ZHANG Xiao-ping and ZHAO Ke.Isolation and screening of deodorant bacterial strains from chicken manure and analysis of their deodorant effects[J].Journal of Agro-Environment Science( J. Agro-Environ. Sci.),2020,39(5):1103-1110. |
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| Authors: | LI Yue LI Cheng-cheng LI Jing ZHANG Xiao-yue ZHANG Zi-wei HUANG Yi-man LIU Hao LUO Hong ZHANG Xiao-ping and ZHAO Ke |
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| Institution: | College of Resources, Sichuan Agricultural University, Chengdu 611130, China,College of Resource and Environment, China Agricultural University, Beijing 100094, China,College of Resources, Sichuan Agricultural University, Chengdu 611130, China,College of Resources, Sichuan Agricultural University, Chengdu 611130, China,College of Resources, Sichuan Agricultural University, Chengdu 611130, China,Wangcang County Agricultural and Rural Bureau, Guangyuan 628200, China,Modern Agricultural Development Promotion Center, Luzhou City Agricultural and Rural Bureau, Luzhou 646000, China,College of Resources, Sichuan Agricultural University, Chengdu 611130, China,College of Resources, Sichuan Agricultural University, Chengdu 611130, China and College of Resources, Sichuan Agricultural University, Chengdu 611130, China |
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| Abstract: | In order to discover strains possessing deodorant effects, which can be used in the harmless treatment of chicken manure, deodorizing bacteria were isolated from chicken manure, and their complexes were constructed. Deodorizing bacteria were screened using several methods:enrichment culture, a plate dilution method, gas production test, ammonia removal test, hydrogen sulfide removal test, and cellulose degradation test. Isolates were then identified by 16S rRNA gene sequencing. Finally, the complex microbial systems were constructed, based on the results of the antagonism effect among strains. Five strains possessing deodorizing functions were isolated; these were Bacillus sp. (MS03), Bacillus velezensis (MS07), Brevibacterium frigoritolerans (MS11), Staphylococcus xylosus (MS42)and Corynebacterium variabile (MS82). The results show that the deodorizing efficiencies of complexes C2 (MS03+MS07)and C4 (MS03+MS82)were higher than 60%, and significantly higher than that for any single bacteria, or for other complexes. Our results indicate that complexes C2 and C4 both have great potential for the harmless treatment of chicken manure. |
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| Keywords: | chicken manure deodorant microorganisms isolation and screening deodorizing effect complex microbial system |
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