| 猪流行性腹泻免疫抗体的ELISA检测及其与琼脂扩散试验相关性分析 |
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| 引用本文： | 潘孝成,芮聪杰,沈学怀,赵瑞宏,戴银,胡晓苗,侯宏艳,周学利,张丹俊.猪流行性腹泻免疫抗体的ELISA检测及其与琼脂扩散试验相关性分析[J].养猪,2019(3):121-123. |
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| 作者姓名： | 潘孝成 芮聪杰 沈学怀 赵瑞宏 戴银 胡晓苗 侯宏艳 周学利 张丹俊 |
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| 作者单位： | 安徽省农业科学院畜牧兽医研究所;安徽省畜禽疫病研究中心;安徽农业大学动物科技学院 |
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| 基金项目： | 安徽省科技重点研发(1704a07020066);安徽省农科院平台项目(2019YL065);安徽省农科院创新团队(13C0405);安徽省生猪产业技术体系项目(AHCYTX-05-09);安徽省科技重大专项(17030701008) |
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| 摘 要： | 为了解不同免疫程序对母猪初乳中猪流行性腹泻病毒(PEDV)抗体水平的影响,以及PEDV抗体的ELISA与琼脂扩散试验(琼扩试验)检测相关性,选取5家免疫程序不同的大型猪场,用PEDV IgA抗体ELISA检测试剂盒对其初乳抗体水平进行检测,并根据检测结果,选OD值大小不同的5组初乳样本,琼扩法检测其PEDV抗体效价并分析。结果显示,5家猪场初乳PEDV IgA抗体阳性率均较高,阳性率为94.00%~100%,阳性样本平均OD值为1.858 8~2.148 0,差异不显著;PEDV抗体琼扩几何平均效价与ELISA检测的OD值存在正相关性。分析表明,母猪产前进行猪流行性腹泻活疫苗和灭活苗各一次的组合免疫较为合理;对PEDV抗体检测琼扩法一定程度上可做为ELISA法的替代。
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| 关 键 词： | 猪流行性腹泻 初乳 免疫抗体 免疫球蛋白A |
ELISA Detection of Anti-swine Epidemic Diarrhea Virus Immune Antibody and Its Correlation with Agar Diffusion Test |
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| PAN Xiaocheng,RUI Congjie,SHEN Xuehuai,ZHAO Ruihong,DAI Yin,HU Xiaomiao,HOU Hongyan,ZHOU Xueli,ZHANG Danjun.ELISA Detection of Anti-swine Epidemic Diarrhea Virus Immune Antibody and Its Correlation with Agar Diffusion Test[J].Swine Production,2019(3):121-123. |
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| Authors: | PAN Xiaocheng RUI Congjie SHEN Xuehuai ZHAO Ruihong DAI Yin HU Xiaomiao HOU Hongyan ZHOU Xueli ZHANG Danjun |
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| Institution: | (Institute of Animal Husbandry and Veterinary Science,Anhui Academy of Agricultural Science,Hefei 230031 China;Livestock and Poultry Epidemic Diseases Research Center of Anhui Province,Hefei 230031China;College of Animal Science and Technology,Anhui Agricultural University,Hefei 230061,China) |
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| Abstract: | PAN Xiaocheng;RUI Congjie;SHEN Xuehuai;ZHAO Ruihong;DAI Yin;HU Xiaomiao;HOU Hongyan;ZHOU Xueli;ZHANG Danjun(Institute of Animal Husbandry and Veterinary Science,Anhui Academy of Agricultural Science,Hefei 230031 China;Livestock and Poultry Epidemic Diseases Research Center of Anhui Province,Hefei 230031China;College of Animal Science and Technology,Anhui Agricultural University,Hefei 230061,China) |
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| Keywords: | swine epidemic diarrhea colostrum immune antibody immunoglobulin A |
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