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| 农杆菌介导西瓜转葡聚糖酶及几丁质酶双基因 | |
| 引用本文： | 张明方,于天祥,杨景华,毛碧增,何祖华.农杆菌介导西瓜转葡聚糖酶及几丁质酶双基因[J].果树学报,2006,23(3):475-478,i0002. |
| 作者姓名： | 张明方 于天祥 杨景华 毛碧增 何祖华 |
| 作者单位： | 1. 浙江大学农业与生物技术学院园艺系,农业部园艺植物生长发育与生物技术重点实验室,杭州,310029 2. 浙江大学农业与生物技术学院生物技术研究所,杭州,310029 3. 中国科学院上海生命科学院植物生理生态研究所,上海,200032 |
| 摘 要： | 采用根癌农杆菌(Agrobacteriumtumefaciens)介导法将含有葡聚糖酶基因和几丁质酶基因表达载体遗传转化西瓜(Citrulluslanatus),遗传转化体系为以子叶为外植体经过组织培养获得再生植株。结果表明,Kan抗性芽的分化率随着共培养时间的延长先上升后下降,GUS阳性芽的百分率逐渐升高,共培养时间30h后,GUS阳性芽的百分率趋于平稳,同时,通过对候选转基因植株中GUS基因染色鉴定、卡那基因、葡聚糖酶基因及几丁质酶基因特异引物PCR检测,表明葡聚糖酶和几丁质酶基因已成功导入西瓜植株中,转基因植株的表现在进一步研究中。 |
| 关 键 词： | 西瓜 葡聚糖酶基因 几丁质酶基因 转基因 |
| 文章编号： | 1009-9980（2006）03-475-04 |
| 收稿时间： | 2005-10-28 |
| 修稿时间： | 2005-10-282006-03-23 |
Transformation of glucanase and chitinase genes to watermelon mediated by Agrobacterium tumefaciens |
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| ZHANG Ming-fang,YU Tian-xiang,YANG Jing-hua,MAO Bi-zeng,HE Zu-hua.Transformation of glucanase and chitinase genes to watermelon mediated by Agrobacterium tumefaciens[J].Journal of Fruit Science,2006,23(3):475-478,i0002. | |
| Authors: | ZHANG Ming-fang YU Tian-xiang YANG Jing-hua MAO Bi-zeng HE Zu-hua |
| Institution: | 1 Department of Horticulture, Zhejiang University, Hangzhou, Zhejiang 310029 China; 2 Insititue of Biotechnology, Zhejiang University, Hangzhou, Zhejiang 310029 China; 3 National Laboratory of Plant Molecular Genetics, Shanghai Institute of Plant Physiology and Ecology, Shanghai Institutes for Biological Sciences, Chinese Academy of Sciences, Shanghai 200032 China |
| Abstract: | A constitute expression vector containing a rice chitinase gene and an alfalfa glucanase gene was transferred into watermelon mediated by Agrobacterium tumefaciens. Planflets were regenerated in vitro by resistance selection on MS medium containing various concentrations of kanamycin, h was shown that the regeneration rate of shoots of anfi-kanamycin was firstly increased and then decreased depended on co-cuhure duration. Furthermore, the positive rate of shoots for GUS staining was increased gradually depended on co-culture duration, and was stable after 30 h of co-culture duration. Meanwhile, GUS staining and PCR-aided detection indicated that both chitinase and glucanase genes had been successfully transferred into watermelon. |
| Keywords: | Watermelon Glucanase Chitinase Gene transformation |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |