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天竺桂叶精油的含量动态、化学成分及体外抗菌活性
引用本文:黄晓冬,黄晓昆,张娴,蔡建秀.天竺桂叶精油的含量动态、化学成分及体外抗菌活性[J].中国农学通报,2010,26(4):182-188.
作者姓名:黄晓冬  黄晓昆  张娴  蔡建秀
作者单位:1. 泉州师范学院,福建泉州,362000
2. 中国科学院城市环境研究所,福建厦门,361000
基金项目:福建省教育厅科技项目 
摘    要:探明天竺桂叶精油含量的月变化动态及高含油量月份的叶精油化学成分组成与抗菌效能。以水蒸汽蒸馏法提取天竺桂叶精油,GC-MS-DS分析叶精油化学成分,纸片扩散法与梯度浓度MIC法测定与评价叶精油的体外抗菌活性。不同月份的天竺桂叶精油含量变化范围约0.25~0.6mL/100g干叶,其中12月份精油含量相对较高,该月份的叶精油主要化学成分为匙叶桉油烯醇(13.522%)、丁香烯(10.403%)、丁香烯氧化物(8.691%)、α,α-4-三甲基-3-环己烯-1-甲醇(8.293%)等,体外抗菌试验表明该精油对普通变形杆菌、藤黄八叠球菌、甘蓝黑腐菌、白菜软腐菌等均具有较强的抑菌活性。天竺桂的适宜采叶期为12月份,此时精油得率较高,抗菌活性也强,在植物源抗菌剂与农药领域具有潜在的开发利用价值。

关 键 词:天竺桂  精油  含量动态  化学成分  抗菌活性
收稿时间:2009/9/27 0:00:00
修稿时间:2009/10/22 0:00:00

Dynamic Content Chemical Composition and Antibacterial Activities of the Essential Oil from Cinnamomum japonicum Leaves
Huang Xiaodong,Huang Xiaokun,Zhang Xian,Cai Jianxiu.Dynamic Content Chemical Composition and Antibacterial Activities of the Essential Oil from Cinnamomum japonicum Leaves[J].Chinese Agricultural Science Bulletin,2010,26(4):182-188.
Authors:Huang Xiaodong  Huang Xiaokun  Zhang Xian  Cai Jianxiu
Institution:Huang Xiaodong1,Huang Xiaokun1,Zhang Xian2,Cai Jianxiu1(1Quanzhou Normal College,Quanzhou Fujian 362000,2Institute of Urban Environment,Chinese Academy of Sciences,Xiamen Fujian 361000)
Abstract:To study the dynamic content of essential oil from Cinnamomum japonicum leaves in different month,the chemical compositions and antibacterial activities of essential oil from the period when it’s content was the highest.The essential oil was extracted by steam distillation and the compositions of essential oil was analyzed by GC-MS-DS,its antibacterial activities were determined by disk diffusion and E-test(MIC)methods.The essential oil compositions of Cinnamomum japonicum dry leaves from different month changed in the range 0.25-0.6 mL/100g and was the most in December,the main components of essential oil in December was Spathulenol(13.522%),Caryophyllene(10.403%),Caryophyllene oxide(8.691%),3-Cyclohexene-1-methanol,alpha.alpha.4-trimethyl-(8.293%),the strong antibacterial activities against Proteus vulgaris、Sarcina lutea、Xanthomonas campestris、Erwinia carotorora were also discovered.The leaves must be picked in December when the essential oil was the most and displayed stong antibacterial activities;it indicated that these essential oils had potential exploitation and utilization value in botanical antimicrobial and botanical pesticide fields.
Keywords:Cinnamomum japonicum  essential oil  dynamic content  chemical constituent  antibacterial activity  
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