| 补体结合酶联免疫吸附试验方法的建立 |
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| 引用本文： | 毛开荣.补体结合酶联免疫吸附试验方法的建立[J].中国兽药杂志,2011,45(11):4-8. |
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| 作者姓名： | 毛开荣 |
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| 作者单位： | 中国兽医药品监察所,北京,100081 |
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| 基金项目： | 公益性行业（农业）项目（200903027），北京市自然科学基金资助项目（6101002） |
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| 摘 要： | 为改进免疫学诊断技术的准确性,研究了一种基于补体结合的免疫学检测新技术———补体结合酶联免疫吸附试验（CF-ELISA）。CF-ELISA技术采用酶标记抗菊糖纯化豚鼠补体C3抗体及其酶显色系统作为补体参与反应的指示系统,用ELISA方法进行补体结合试验。经对布氏菌病抗体检测的初步试验结果显示,CF-ELISA技术可检测到0.01 IU的布氏菌病抗体,灵敏度与间接酶联免疫吸附试验（iELISA）相当,是虎红平板凝集试验（RBPT）试管凝集试验（SAT）的5 000倍、补体结合试验（CFT）的10 000倍。对349份确诊布氏菌病感染群牛、羊血清的检测结果显示,CF-ELISAi、ELISA、CFT、SAT、RBPT的阳性率分别为35.82%3、6.39%、31.81%、30.09%、36.1%,CF-ELISA与iELISA、CFT、SAT、RBPT的阳性符合率分别为：98.4%、88.8%、80.0%、90.6%。CF-ELISAi、ELISA、CFT、SAT、RBPT对490份布氏菌病阴性群牛、羊血清的阴性率分别为100%、99.6%、100%、99.4%、99.8%,CF-ELISA与iELISA、CFT、SAT、RBPT的阴性符合率分别为：99.6%1、00%、99.4%、99.8%。研究表明,CF-ELISA是具有高特异性和高敏感性的布氏菌病免疫学检测技术。
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| 关 键 词： | CF-ELISA 布氏菌病 敏感性 特异性 |
| 收稿时间： | 2011/10/26 0:00:00 |
| 修稿时间： | 2011/10/26 0:00:00 |
Establishment of Complement Fixation-Enzyme Linked Immunosorbent Assay (CF-ELISA) for the Detection of Brucellosis |
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| MAO Kai-rong.Establishment of Complement Fixation-Enzyme Linked Immunosorbent Assay (CF-ELISA) for the Detection of Brucellosis[J].Chinese Journal of Veterinary Drug,2011,45(11):4-8. |
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| Authors: | MAO Kai-rong |
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| Institution: | China Institute of Veterinary Drug Control |
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| Abstract: | A new complement fixation-enzyme linked immunosorbent assay（CF-ELISA）method was established to improve accuracy rate of immunological test.CF-ELISA was test used ELISA mothed,that antibody conjugated Horse Radish Peroxidase（HRP） is anti-complement C3 extracted and purified with inulin from the serum of guinea pigsand the enzyme-substrate system be used detect complement fixation with antibody-antigen complex.The CF-ELISA method could detect 0.01 IU antibody,which had the same sensitivity with iELISA,and about 5 000 times higher than that of RBPT、SAT for brucellosis.The positive rate of 349 serum samples from Brucella-infected flocks of goats/sheep and bovines with CF-ELISA,iELISA,CFT,SAT and RBPT method was 35.82%,36.39%,31.81%,30.09% and 36.1%,respectively.The positive coincidence rate of CF-ELISA to iELISA,CFT,SAT,RBPT was 98.4%,88.8%,80.0% and 90.6%.The negative rate of 490 samples collected from Brucella-free flocks with CF-ELISA,iELISA,CFT,SAT and RBPT was 100%,99.6%,100%,99.4% and 99.8%,respectively.The negative coincidence rate of CF-ELISA to iELISA,CFT,SAT,RBPT was 99.6%,100%,99.4% and 99.8%.The results showed that CF-ELISA was a promising candidate method for the detection of brucellosis with high specificity and sensitivity. |
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| Keywords: | complement fixation-enzyme linked immunosorbent assay (CF-ELISA) brucellosis specificity sensitivity |
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