| 基于微卫星标记对6个花鲈群体的遗传多样性分析 |
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| 引用本文： | 黄皓,范嗣刚,王鹏飞,陈佳,赵超,闫路路,邱丽华,潘滢.基于微卫星标记对6个花鲈群体的遗传多样性分析[J].南方水产科学,2022,18(1):99-106. |
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| 作者姓名： | 黄皓 范嗣刚 王鹏飞 陈佳 赵超 闫路路 邱丽华 潘滢 |
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| 作者单位： | 上海海洋大学 水产与生命学院,上海 201306;中国水产科学研究院南海水产研究所/广东省渔业生态环境重点实验室,广东 广州 510300;中国水产科学研究院南海水产研究所/广东省渔业生态环境重点实验室,广东 广州 510300;宁德市富发水产有限公司/大黄鱼育种国家重点实验室,福建 宁德 352103 |
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| 基金项目： | 国家自然科学基金项目(31802281);广东省自然科学基金项目(2018A030313137);广州市珠江科技新星项目(201906010028);中国水产科学研究院中央级公益性科研院所基本科研业务费专项资金资助(2020XT09,2020TD21);中国水产科学研究院南海水产研究所中央级公益性科研院所基本科研业务费专项资金资助(2021SD11)。 |
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| 摘 要： | 为了解中国不同地理群体花鲈(Lateolabrax maculatus)的遗传结构,从花鲈基因组序列中筛选出11个具有多态性的微卫星位点,对中国沿海地区(天津、长岛、青岛、上海、厦门和北海)6个花鲈野生群体的遗传多样性进行分析.11个微卫星位点共检测到57个等位基因,7个微卫星位点具有高度多态性.6个群体的等位基因数(...
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| 关 键 词： | 花鲈 微卫星 遗传多样性 遗传分化 |
Genetic diversity analysis of six geographical populations of Lateolabrax maculatus based on microsatellite markers |
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| HUANG Hao,FAN Sigang,WANG Pengfei,CHEN Jia,ZHAO Chao,YAN Lulu,QIU Lihua,PAN Ying.Genetic diversity analysis of six geographical populations of Lateolabrax maculatus based on microsatellite markers[J].South China Fisheries Science,2022,18(1):99-106. |
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| Authors: | HUANG Hao FAN Sigang WANG Pengfei CHEN Jia ZHAO Chao YAN Lulu QIU Lihua PAN Ying |
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| Institution: | (College of Fisheries and Life Science,Shanghai Ocean University,Shanghai 201306,China;South China Sea Fisheries Research Institute,Chinese Academy of Fishery Sciences/Guangdong Provincial Key Laboratory of Fishery Ecology and Environment,Guangzhou 510300,China;State Key Laboratory of Large Yellow Croaker Breeding,Ningde Fufa Fisheries Co.Ltd.,Ningde 352103,China) |
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| Abstract: | In order to analyze the genetic structure of Lateolabrax maculatus, we selected 11 polymorphic microsatellite loci from L. maculatus genome sequence to investigate the genetic structure of six wild populations of L. maculatus. All of the wild L. maculatus were fished from the coast of Tianjin, Qingdao, Changdao, Shanghai, Xiamen and Beihai, China, respectively. A total of 57 alleles were detected from 11 polymorphic microsatellite loci and 7 microsatellite loci were highly polymorphic loci.Among the six populations, the number of alleles(N;) was 3.909 3–4.636 4 and the effective number of alleles(N;) was 2.293 4–2.773 5. The observed heterozygosity(H;) was 0.391 3–0.456 8 and the expected heterozygosity(H;) was 0.505 1–0.566 2. The polymorphism information content(PIC) was 0.388 8–0.518 9. The populations of L. maculatus from Qingdao, Shanghai and Beihai had highly polymorphism, and the other populations had moderate polymorphism. The Shanghai population had the highest polymorphism among all the populations. Changdao and Xiamen populations had low polymorphism. The genetic differentiation index(F;) was 0.022 6–0.055 2. The genetic differentiation among the six population was low and the highest genetic differentiation was detected between Tianjin population and Beihai population. The gene flow(N;) was 4.276 6–11.220 8,and the most frequent gene exchange was found among these populations. The analysis of molecular variance(AMOVA) shows that the variation among populations accounted for 91% and the variation within populations accounted for 9%. The cluster analysis based on individual classification shows that the individuals of six populations were divided into two genotype groups and no independent genotype group was detected. The UPMGA cluster tree based on genetic distance shows that six populations were divided into two branchs. |
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| Keywords: | Lateolabrax maculatus Microstatelites Genetic diversity Genetic differentiation |
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