| 香蕉系统获得抗性相关基因对外源水杨酸的响应分析 |
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| 引用本文： | 段雅婕,郭志祥,曾莉,李舒,刘立娜,胡会刚,李伟明,白亭亭.香蕉系统获得抗性相关基因对外源水杨酸的响应分析[J].热带作物学报,2022,43(1):9-18. |
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| 作者姓名： | 段雅婕 郭志祥 曾莉 李舒 刘立娜 胡会刚 李伟明 白亭亭 |
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| 作者单位： | 1.中国热带农业科学院南亚热带作物研究所/农业农村部热带果树生物学重点实验室/海南省热带园艺产品采后生理与保鲜重点实验室,广东湛江 5240912.云南省农业科学院农业环境资源研究所,云南昆明 650205 |
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| 基金项目： | 财政部和农业农村部国家现代农业产业技术体系项目(No.CARS-31-08);西藏自治区自然科学基金项目(No.XZ 2018ZR G-46);广东省自然科学基金项目(No.2020A1515010166)。 |
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| 摘 要： | 香蕉生产受多种病虫害和逆境胁迫的影响,由真菌病原引起的香蕉枯萎病、叶斑病和黑星病,细菌性病害软腐病和鞘腐病,以及非生物胁迫寒害等,是阻碍香蕉绿色可持续生产的严重问题.为探索香蕉生产上多种病害和寒害逆境的有效防控措施,本研究从外源水杨酸(SA)诱导植物系统抗性机理出发,通过实时荧光定量PCR(RT-qPCR)方法,分析外...
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| 关 键 词： | 香蕉 诱导抗性 抗性基因 生物与非生物胁迫 |
| 收稿时间： | 2021-04-12 |
Analysis of Expression of Systemic Acquired Resistance-related Genes in Banana Induced by Exogenous Salicylic Acid |
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| DUAN Yajie,GUO Zhixiang,ZENG Li,LI Shu,LIU Lina,HU Huigang,LI Weiming,BAI Tingting.Analysis of Expression of Systemic Acquired Resistance-related Genes in Banana Induced by Exogenous Salicylic Acid[J].Chinese Journal of Tropical Crops,2022,43(1):9-18. |
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| Authors: | DUAN Yajie GUO Zhixiang ZENG Li LI Shu LIU Lina HU Huigang LI Weiming BAI Tingting |
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| Institution: | 1. South Asia Tropical Crops Research Institute, Chinese Academy of Tropical Agriculture Sciences / Key Laboratory of Tropical Fruit Biology, Ministry of Agriculture and Rural Affairs / Key Laboratory of Hainan Province for Postharvest Physiology and Technology of Tropical Horticultural Products, Zhanjiang, Guangdong 524091, China2. Agricultural Environment and Resources Institute, Yunnan Academy of Agricultural Sciences, Kunming, Yunnan 650205, China |
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| Abstract: | Banana production is affected by a variety of diseases, pests and abiotic stresses. Banana Fusarium wilt, sigatoka and black spot caused by fungal pathogens, soft rot and sheath rot caused by bacteria, abiotic stresses such as cold damage, are serious problems hindering banana green sustainable production. In order to explore effective prevention and control measures for a cultivar of diseases and cold stress in banana production, this study started from the mechanism of exogenous salicylic acid inducing plant system resistance, and analyzed the induced expression of exogenous salicylic acid (SA) on banana systemic resistance-related genes by the real-time fluorescent quantitative PCR (RT-qPCR) method. Results showed that exogenous SA could induce significant up-regulation of key genes in the SA synthesis pathway in banana plants. The relative expression level of SK gene in SA-treated susceptible ‘Brazilian’ was more than 1.5 folds than that in the control ‘Brazilian’, while the relative expression level of this gene in SA-treated tolerant ‘Nongke No. 1’ was more than 30 folds than that in the control ‘Nongke No. 1’. The PAL genes showed a significant up-regulation trend in both of SA-treated ‘Brazilian’ and ‘Nongke No. 1’. In the SA-treated ‘Nongke No. 1’, the highest significantly up-regulated expression of PAL was 12.5 folds, and the lowest significantly up-regulated expression was 1.4 folds. While in SA-treated ‘Brazilian’, the highest significantly up-regulated expression of PAL was 3.1 folds and the lowest significantly up-regulated expression was 1.7 folds. The up-regulation of SK and PAL genes in the tolerant cultivar ‘Nongke No. 1’ treated with SA was much higher than that in the susceptible cultivar ‘Brazilian’ treated with SA. SA induced up-regulation of CS and ICS genes by no more than 3 folds, but it still showed that the up-regulation in the tolerant cultivar ‘Nongke No. 1’ was higher than that in the susceptible cultivar ‘Brazilian’. NPR1, TGA, and PR1 genes were induced significantly up-regulated in both two cultivars. And ‘Nongke No. 1’ had a more obvious induction effect than ‘Brazilian’. The response of TR4-resistance genes in SA-treated bananas was also determined. Results showed that TR4 inoculation for 3 days could inhibit the expression of most PAL, NPR1 and PR1 genes in ‘Brazilian’, but not in ‘Nongke No. 1’. Under the dual treatment of SA and TR4, the TR4-resistance genes of PAL, NPR1 and PR1 in the two banana cultivars were strongly induced to be up-regulated. The activities of PAL and POD defense enzymes were significantly enhanced. This indicated that exogenous SA has the potential to induce systemic resistance in bananas against a variety of biotic and abiotic stresses. This research provides a theoretical basis for the comprehensive prevention and control of various diseases in banana production and the improvement of banana resistance. |
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| Keywords: | banana induced resistance resistant gene biotic and abiotic stresses |
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