| 油棕脂肪酸外运蛋白基因FAX1的克隆及表达分析 |
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| 引用本文： | 金龙飞,李睿,曹红星,颜彦.油棕脂肪酸外运蛋白基因FAX1的克隆及表达分析[J].热带作物学报,2022,43(1):27-33. |
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| 作者姓名： | 金龙飞 李睿 曹红星 颜彦 |
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| 作者单位： | 1.中国热带农业科学院椰子研究所/海南省热带油料作物生物学重点实验室,海南文昌 5713392.中国热带农业科学院热带生物技术研究所/农业农村部热带作物生物学与资源利用重点实验室,海南海口 571101 |
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| 基金项目： | 中国热带农业科学院基本科研业务费专项资金(No.1630052021028,No.1630152021004,No.1630152017004)。 |
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| 摘 要： | 油棕(Elaeis guineensis Jacq.)原产非洲,是重要的热带木本油料作物,产油效率极高.目前油棕广泛种植在东南亚、非洲、中南美洲和我国的海南、云南、广东、广西等省(区),油棕果实压榨的棕榈油是重要的食用油和工业原料.脂肪酸在植物质体中合成,然后转运到内质网上进行加工和修饰.为了研究油棕脂肪酸的转运机制,...
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| 关 键 词： | 脂肪酸 FAX1 油棕 生物信息学 基因表达 |
| 收稿时间： | 2021-05-10 |
Identification and Expression Analysis of Fatty Acid Export Gene FAX1 in Oil Palm (Elaeis guineensis) |
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| JIN Longfei,LI Rui,CAO Hongxing,YAN Yan.Identification and Expression Analysis of Fatty Acid Export Gene FAX1 in Oil Palm (Elaeis guineensis)[J].Chinese Journal of Tropical Crops,2022,43(1):27-33. |
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| Authors: | JIN Longfei LI Rui CAO Hongxing YAN Yan |
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| Institution: | 1. Coconut Research Institute of Chinese Academy of Tropical Agricultural Science / Hainan Key Laboratory of Tropical Oil Crops Biology, Wenchang, Hainan 571339, China2. Institute of tropical Bioscience and Biotechnology, Chinese Academy of Tropical Agricultural Sciences / Key Laboratory of Biology and Genetic Resources of Tropical Crops, Ministry of Agriculture & Rural Affairs, Haikou, Hainan 571101, China |
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| Abstract: | Oil palm (Elaeis Guineensis Jacq.), originated in Africa, is an important tropical woody oil crop with high oil production efficiency. It is widely planted in Southeast Asia, Africa, Central and South America and Hainan, Yunnan, Guangdong, Guangxi of China. Palm oil squeezed from oil palm fruits is an important edible oil and industrial raw material. Fatty acids are synthesized in plant plastids and then transported into the endoplasmic reticulum for processing and modification. In order to study the transport mechanism of fatty acids in oil palm, the key genes controlling transportion of fatty acids were extracted by bioinformatics and fluorescence quantitative PCR using ‘Reyou 4’ oil palm. Fatty acid export 1 (FAX1) is a plant fatty acid transporter, which mediates the fatty acids export in plastids, and plays an important role in the regulation of lipid biosynthesis. In this study, three EgFAX1 genes (EgFAX1-1, EgFAX1-2, EgFAX1-3) were cloned from oil palm, and the amino acid length, protein instability index, aliphatic coefficient, the total mean hydrophilicity, chromosomal localization, gene structure, conserved functional domain, evolutionary relationship and expression characteristics were analyzed. The amino acid length of the peptide of EgFAX1 was 189, 231 and 232. The molecular weight was 20.43, 24.85 and 24.92 kDa. The isoelectric point was 9.82, 9.82 and 9.93. The protein instability index was 48.01, 49.71 and 50.09. The aliphatic coefficient was 81.06, 84.07 and 83.28. The total mean hydrophilicity was 0.071, 0.121 and 0.106, respectively. Sequence and amino acid domain characterization analysis showed that EgFAX1 contained 2, 5 and 7 exons, and contained Tmemb_14 protein domain. Phylogenetic analysis of FAX1 in oil palm, Arabidopsis, rice, soybean, rapeseed, tomato, green algae, red algae, Gonium pectorale, Volvox carteri, Ectocarpus siliculosus, Micromonas pusilla and Chlorella variabilis showed that oil palm was closely related to soybean and tomato. The gene expression characteristics analysis in root, shoot, leaf, flower and fruit showed that EgFAX1 was highly expressed in flowers and fruits. The gene expression characteristics analysis in different fruit development stage including 15 weeks, 17 weeks, 21 weeks and 23 weeks after anthesis (WAA) showed that the expression level of three EgFAX1 increased during fruit development and then decreased gradually after reaching the peak value. The expression level of EgFAX1-1 peaked at 17 WAA and the expression level of EgFAX1-2 and EgFAX1-3 peaked at 21 WAA. The results would provide useful information for further investigation mechanism of EgFAX1 regulating fatty acid transportation in oil palm. |
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| Keywords: | fatty acid FAX1 oil palm bioinformatics gene expression |
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