| 三倍体虹鳟实时荧光定量PCR内参基因稳定性分析 |
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| 引用本文： | 苏晓燕,韩步鹰,孟玉琼,白晓易,李长忠,马睿.三倍体虹鳟实时荧光定量PCR内参基因稳定性分析[J].水产科学,2022,41(1):35-43. |
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| 作者姓名： | 苏晓燕 韩步鹰 孟玉琼 白晓易 李长忠 马睿 |
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| 作者单位： | 青海大学生态环境工程学院,青海西宁810016;省部共建三江源生态与高原农牧业国家重点实验室,青海西宁810016;省部共建三江源生态与高原农牧业国家重点实验室,青海西宁810016;青海大学生态环境工程学院,青海西宁810016 |
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| 基金项目： | 国家自然基金项目资助项目(31860731);青海省科技厅项目(2019-NK-104). |
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| 摘 要： | 采用实时荧光定量PCR(qRT-PCR)方法比较甘油醛-3-磷酸脱氢酶(gapdh)、核糖体大亚基蛋白基因(rplp2)、真核延伸因子基因(ef1-α)、β-肌动蛋白(β-actin)和18S核糖体核酸基因(18S rRNA)等5个候选内参基因在体质量约800 g三倍体虹鳟脑、眼、鳃、皮肤、心脏、肾脏(头肾、中肾、后肾...
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| 关 键 词： | 三倍体虹鳟 实时荧光定量PCR 内参基因 |
Stability of Reference Genes in Different Tissues of Triploid Rainbow Trout Oncorhy nchus my kiss by Quantitative Real-Time PCR |
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| SU Xiaoyan,HAN Buying,MENG Yuqiong,BAI Xiaoyi,LI Changzhong,MA Rui.Stability of Reference Genes in Different Tissues of Triploid Rainbow Trout Oncorhy nchus my kiss by Quantitative Real-Time PCR[J].Fisheries Science,2022,41(1):35-43. |
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| Authors: | SU Xiaoyan HAN Buying MENG Yuqiong BAI Xiaoyi LI Changzhong MA Rui |
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| Institution: | (College of Eco-Environmental Engineering, Qinghai University, Xining 810016, China;State Key Laboratory of Plateau Ecology and Agriculture, Xining 810016, China) |
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| Abstract: | Five candidate internal reference genes including gapdh(glyceraldehydes-3-phosphate dehydrogenase),ribosomal protein genes 2(rplp2),elongation factors1-α(ef1-α),beta-actin(β-actin),and 18S ribosomal RNA(18S rRNA)were comparatively expressed in brain,eyes,gills,skin,heart,kidney(head kidney,mid-kidney,and metanephros),liver,spleen,stomach,pyloric caeca,intestine(foregut,midgut,and hindgut)and muscle(red muscle,white muscle,and intermuscular compartment)of triploid rainbow trout Oncorhynchus mykiss with body weight of about 800 g by Real-time fluorescent quantitative PCR(qRT-PCR)method to screen stable reference genes in different tissues of triploid rainbow trout.The expression stability of the 5 candidate internal reference genes were analyzed by comparing the cycle threshold(Ct value)of the internal reference gene expression,and by three softwares including GeNorm,Best-Keeper,and NormFinder.The GeNorm analysis showed that the descending order of the M value was described asβ-actin=rplp2
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| Keywords: | triploid rainbow trout Oncorhynchus mykiss quantitative real-time PCR(qRT-PCR) reference gene |
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