| Assessment of the Genetic Diversity of Pummelo Germplasms Using AFLP and SSR Markers |
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| 作者姓名： | LIU Yong SUN Zhong-hai LIU De-chun WU Bo TAO Jian-jun |
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| 作者单位： | [1]Citrus Research Institute, Huazhong Agricultural University, Wuhan 430070, P.R.China [2]College of Agronomy, Jiangxi Agricultural University Nanchang 330045, P.R.China |
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| 基金项目： | 国家自然科学基金，高等学校优秀青年教师教学科研奖励计划，Funds of The Citrus Research Institute;Chinese Academy of Agricultural Sciences，Pummelo Germplasms Repository of Meixian County，Guangdong Province for providing part of plant materials |
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| 摘 要： |
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| 关 键 词： | 柚子 AFLP SSR 基因多样性 种质资源 分子标记 |
Assessment of the Genetic Diversity of Pummelo Germplasms Using AFLP and SSR Markers |
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| LIU Yong SUN Zhong-hai LIU De-chun WU Bo TAO Jian-jun.Assessment of the Genetic Diversity of Pummelo Germplasms Using AFLP and SSR Markers[J].Agricultural Sciences in China,2005,4(10):733-740. |
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| Authors: | LIU Yong SUN Zhong-hai LIU De-chun WU Bo TAO Jian-jun |
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| Abstract: | The genetic diversities of 110 pummelo germplasms and 12 of their relatives were analyzed by SSR and AFLP methods. Approximately 99.1% of the 335 SSR loci were polymorphic, and 9.85 alleles per SSR locus were identified. The gene diversity values changed from 0.1939 to 0.9073, and 46 SSR polymorphic bands were scored. 72% of the 343 AFLP loci were polymorphic, and 82 polymorphic loci per AFLP were identified. Heterozygosity changed from 0.21863 to 0.28445, and 44 AFLP polymorphic bands were scored. The UPGMA result showed that 122 pummelo genotypes and their relatives could be divided into eight groups, and the pummelo genotypes composed mainly of Shatian pummelo varieties group, Wendan pummelo vareties group and a huge hybrid pummelo varieties group. The classification result was expected to widen the genetic background of pummelos using various target varieties. |
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| Keywords: | Pummelo Citrus grandis Osbeck Germplasm resource Genetic diversity SSR AFLP |
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