| 禽大肠杆菌O2、O78菌株外膜蛋白A基因扩增及序列分析 |
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| 引用本文： | 曹素芳,黄青云,温纳相,陈红英.禽大肠杆菌O2、O78菌株外膜蛋白A基因扩增及序列分析[J].华南农业大学学报,2004,25(1):99-102. |
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| 作者姓名： | 曹素芳 黄青云 温纳相 陈红英 |
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| 作者单位： | 华南农业大学,兽医学院,广东,广州,510642;华南农业大学,兽医学院,广东,广州,510642;华南农业大学,兽医学院,广东,广州,510642;华南农业大学,兽医学院,广东,广州,510642 |
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| 基金项目： | 广东省自然科学基金资助项目(960445) |
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| 摘 要： | 根据Genbank中大肠杆菌K-12外膜蛋白A基因的核苷酸序列设计引物，从禽大肠杆菌O为O2、O78及它们的融合株O2．78(Chl^rNor^r)中分别扩增得到外膜蛋白A基因(ompA)，进行序列测定及分析发现3个菌株的ompA均由2276nt组成，核苷酸序列完全相同，只有一个大的开放性阅读框(ORF)，长1041bp，编码由346个氨基酸组成的前外膜蛋白A(pro-OmpA)，前21个氨基酸残基组成信号肽，成熟的OmpA由325个氨基酸残基组成，相对分子质量为37000．其氨基酸序列也完全相同．从基因水平上证明了禽大肠杆菌O2和O78菌株存在相同的外膜蛋白A抗原。
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| 关 键 词： | 禽大肠杆菌 外膜蛋白A基因 核苷酸序列分析 氨基酸序列分析 |
| 文章编号： | 1001-411X(2004)01-0099-04 |
Amplified and sequence analysis of the outer membrane protein A gene of avian Escherichia coil O2, O78 |
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| CAO Su-fang,HUANG Qing-yun,WEN Na-xiang,CHEN Hong-ying.Amplified and sequence analysis of the outer membrane protein A gene of avian Escherichia coil O2, O78[J].Journal of South China Agricultural University,2004,25(1):99-102. |
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| Authors: | CAO Su-fang HUANG Qing-yun WEN Na-xiang CHEN Hong-ying |
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| Abstract: | A pair of primers were designed based on the DNA sequence of the outer membrane protein A gene (ompA)in Genbank. With the special primers, ompA of E.coli O_2, O_(78) and their fusion bivalent attenunated strain O_(2,78) (Chl~r Nor~r) were amplified by PCR. After sequencing , all of the three ompA were shown to contain (2 276) nt. Starting at nucleotide (1 037) and ending at nucleotide (2 077), an open reading frame(ORF) of (1 041) bp encodes the pro-OmpA protein consisting of 346 amino acid residues. Hydrophobicity analysis suggested that the preceeding 21 amino acid constitute a typical signal sequence, and that the mature form of OmpA is 325 amino acid long with a relative molecular mass of (37 000). Analysis of the nucleotide sequences of the three ompA genes of avian Escherichia coli showed that they were all identical. These results indicated that O_2 and O_(78) have the same outer membrane protein A antigen. |
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| Keywords: | avian Escherichia coli outer membrane protein A gene nucleotide sequence analysis amino acid sequence analysis |
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