| J亚群禽白血病病毒gp85基因的克隆与表达 |
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| 引用本文： | 贾纯琰,张莉,李永清,张培君.J亚群禽白血病病毒gp85基因的克隆与表达[J].动物医学进展,2008,29(5):7-9. |
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| 作者姓名： | 贾纯琰 张莉 李永清 张培君 |
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| 作者单位： | 1. 首都师范大学生命科学院,北京,100037
2. 北京市农林科学院畜牧兽医研究所,北京,100097 |
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| 摘 要： | 构建J亚群禽白血病病毒gp85基因的原核表达载体,并在大肠埃希菌中获得表达。根据原核表达载体pET30的酶切图谱设计引物,以重组质粒pBS-T-env为模板,扩增gp85基因。将此段基因克隆到表达载体pET30上,转化大肠埃希菌BL21,用IPTG诱导表达,经SDS-PAGE分析,表达出约为70ku的融合蛋白。获得了gp85基因的表达产物,为研制ALV-J的特异性抗血清及国内ALV-J病毒分子诊断试剂盒奠定了基础。
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| 关 键 词： | J-亚群禽白血病病毒 gp85基因 克隆 表达 |
| 文章编号： | 1007-5038(2008)05-0007-03 |
| 修稿时间： | 2008年1月24日 |
Cloning and Expression of gp85 Gene of Avian leukosis virus Subgroup J |
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| JIA Chun-yan,ZHANG Li,LI Yong-qing,ZHANG Pei-jun.Cloning and Expression of gp85 Gene of Avian leukosis virus Subgroup J[J].Progress In Veterinary Medicine,2008,29(5):7-9. |
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| Authors: | JIA Chun-yan ZHANG Li LI Yong-qing ZHANG Pei-jun |
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| Abstract: | To construct the prokaryotic expression vector for gp85 gene of avian leukosis virus subgroup J and express the fusion protein in E.coli.According to the prokaryotic expression vector restriction map pET30,a pair of primers were designed to amplify the gp85 gene and the recombinant plasmid pBS-T-env was used as templates for PCR reaction.The gp85 gene was cloned into the expression vector pET30,transformed in E.coli BL21 and induced by IPTG.SDS-PAGE analysis showed that the fusion protein was about 70 ku in size,indicatin that Obtained the expressive product of the gp85 gene was obtained,it lays a basis for developing the special antiserum of ALV-J and the domestic ALV-J molecular diagnostic reagent. |
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| Keywords: | ALV-J gp85 gene cloning expression |
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