| 桉树Genomic-SSR和EST-SSR分子标记的遗传差异性分析 |
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| 引用本文： | 刘果,谢耀坚,陈鸿鹏,吴志华.桉树Genomic-SSR和EST-SSR分子标记的遗传差异性分析[J].桉树科技,2017,34(3). |
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| 作者姓名： | 刘果 谢耀坚 陈鸿鹏 吴志华 |
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| 作者单位： | 国家林业局桉树研究开发中心,广东 湛江,524022 |
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| 基金项目： | 中央级公益性科研院所基本科研业务费专项资金 |
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| 摘 要： | 为探讨Genomic-SSR与EST-SSR标记在桉树种间的遗传差异,本研究利用两种不同来源的SSR标记对6种桉树进行遗传差异性分析.分析结果表明:Genomic-SSR标记在6种桉树中具有高的遗传多样性,EST-SSR标记在揭示不同基因型间遗传关系方面更具优势.对两种标记进行聚类分析发现,EST-SSR标记比Genomic-SSR标记能更准确地鉴别基因型.两种不同来源SSR标记的遗传差异性,为更有效利用SSR标记在桉树遗传多样性分析、种质鉴定等相关研究提供了理论支持.
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| 关 键 词： | 桉树 Genomic-SSR EST-SSR 遗传差异性 |
Genetic Differences Revealed by Genomic-SSRs and EST-SSRs in Eucalyptus Species |
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| LIU Guo,XIE Yao-jian,CHEN Hong-peng,WU Zhi-hua.Genetic Differences Revealed by Genomic-SSRs and EST-SSRs in Eucalyptus Species[J].Eucalypt Science & Technology,2017,34(3). |
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| Authors: | LIU Guo XIE Yao-jian CHEN Hong-peng WU Zhi-hua |
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| Abstract: | To explore the genetic differences between genomic-SSR and EST-SSR markers in different species of Eucalyptus, the genetic diversity of 6 species were analyzed using two types of SSR markers in the study reported here. Genomic-SSRs were found to be more polymorphic than EST-SSRs in all 6 Eucalyptus species, with EST-SSRsproving to be more powerful for revealing genetic relationships among different genotypes than genomic-SSRs. Moreover, cluster analyses indicated that EST-SSR markers could identify different genotypes more accurately. The genetic differences revealed in this study for genomic-SSR and EST-SSR markers will provide theoretical support for rational future use of SSR markers in studies of Eucalyptus genetic diversity, germplasm identification and other related research. |
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| Keywords: | eucalyptus genomic-SSR EST-SSR genetic differences |
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