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| 应用间接ELISA检测犬冠状病毒抗体 | |
| 引用本文： | 范志强,夏咸柱,胡桂学,黄耕,邹啸环,武银莲,毕森林,张治国.应用间接ELISA检测犬冠状病毒抗体[J].中国兽医学报,1999,19(2):129-132. |
| 作者姓名： | 范志强 夏咸柱 胡桂学 黄耕 邹啸环 武银莲 毕森林 张治国 |
| 作者单位： | 1. 沈阳军区后勤部药品检验所,沈阳,120026 2. 解放军农牧大学军事兽医研究所,长春,130062 3. 沈阳军区后勤部药品检验所 |
| 基金项目： | “九五”军队医药卫生科研基金 |
| 摘 要： | 为了建立一种快速、敏感、特异的检测犬冠状病毒（ＣＣＶ）的方法，利用感染ＣＣＶ的犬肾传代细胞包被酶联反应板，以辣根过氧化物酶标记的金黄色葡萄球菌Ａ蛋白（ＨＲＰ－ＳＰＡ）作为第２抗体，建立了检测ＣＣＶ抗体的间接ＥＬＩＳＡ方法。抗原的包被量为每孔３×１０４个染毒细胞，酶标抗体的工作浓度为１∶４０。试验发现，包被的病毒抗原经甲醇固定３０ｍｉｎ后可以提高试验的敏感性，减少病毒抗原的使用量。与中和试验比较证实，２种抗体检测方法的测定结果呈正相关 |
| 关 键 词： | 犬冠状病毒 间接ELISA 犬 |
| 修稿时间： | 1998-03-26 |
Indirect ELISA for Detecting Serum Antibodies to Canine Coronavirus |
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| Fan Zhiqiang,Xia Xianzhu,Hu Guixue,Huang Geng,Zou Xiaohuan,Wu Yinlian,Bi Senlin,Zhang Zhiguo.Indirect ELISA for Detecting Serum Antibodies to Canine Coronavirus[J].Chinese Journal of Veterinary Science,1999,19(2):129-132. | |
| Authors: | Fan Zhiqiang Xia Xianzhu Hu Guixue Huang Geng Zou Xiaohuan Wu Yinlian Bi Senlin Zhang Zhiguo |
| Abstract: | An enzyme linked immunosorbent assay (ELISA) for detecting serum antibodies to CCV was estabolished by using DK cell grown CCV as antigens for coating. A parallel was drown between the indirect ELISA and virus neutralization (VN) test, the titers obtained in both ways were correlated positively well and indirect ELISA could take or partially take place the VN test in serodiagnosis and serosurveys. In this study it was shown that using DK cell grown CCV as antigens for coating could reduce the non specificity of the test, and that the fixation of antigen in methanol could improve the sensitivity of the test and reduce the concentration of antigens for use. |
| Keywords: | CCV indirect ELISA canine |
| 本文献已被 CNKI 维普 万方数据 等数据库收录！ | |