| 不同生境春季核桃内生真菌分离及其多样性 |
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| 引用本文： | 阿依佳玛丽·依玛尔,刘泽星,毛光瑞,翟梅枝.不同生境春季核桃内生真菌分离及其多样性[J].西北林学院学报,2016,31(6):184-189. |
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| 作者姓名： | 阿依佳玛丽·依玛尔 刘泽星 毛光瑞 翟梅枝 |
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| 作者单位： | （西北农林科技大学 林学院 核桃研究中心，陕西 杨陵 712100） |
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| 摘 要： | 以核桃（Juglans regia）的不同部位（根、1年生茎、2年生茎和干茎）为材料，采用组织分离法，对来自陕西山阳县、蓝田县与宜君县3个不同生境的材料进行内生真菌的分离鉴定、种群组成、定殖率、多样性指数等研究。结果显示，从576块组织块中共分离得到392株内生真菌，其中在2年生茎124株，1年生茎120株，干茎87株，根61株。392株内生真菌隶属于24个属，其中，3大优势类群为交链孢霉属（Alternaria）、花核菌属（Anthina）和茎叶核菌属（Ectostroma），分别占菌株总数的19.90%、9.69%和8.93%。交链孢霉属主要存在于2年生茎和干茎中，花核菌属和茎叶核菌属则主要存在于2年生茎中。核桃不同部位内生真菌多样性指数1年生茎的最高，为1.07；不同生境中，山阳核桃内生真菌的多样性指数最高，为1.30，说明春季核桃内生真菌具有丰富的多样性，是潜在的真菌资源库。
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| 关 键 词： | 核桃 不同生境 内生真菌 生物多样性 |
Isolation and Diversity of Endophytic Fungi from Juglans regia in Spring under Different Habitats |
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| YIMAER·A-yi-jia-ma-li,LIU Ze-xing,MAO Guang-rui,ZHAI Mei-zhi. Isolation and Diversity of Endophytic Fungi from Juglans regia in Spring under Different Habitats[J].Journal of Northwest Forestry University,2016,31(6):184-189. |
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| Authors: | YIMAER·A-yi-jia-ma-li LIU Ze-xing MAO Guang-rui ZHAI Mei-zhi |
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| Institution: | （Walnut Research Centre,College of Forestry,Northwest A&F university,Yangling,Shaanxi 712100,China） |
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| Abstract: | Fungal endophytes of Juglans regia from different habitats and parts were isolated by tissue expanding method.The composition,diversity and preference of fungal endophytes were analyzed by the colonization frequency (CF),isolation rate (IR),isolation frequency (IF),and Shannon-Wiener biodiversity index (H′).Totally,392 fungal endophytes (61 strains in roots,120 strains in 1-year-old twig,124 strains in 2-year-old twig and 87 strains in scapus) were isolated and classified into 24 genera.Among them,Alternaria (19.90%),Anthina (9.69%),Ectostroma (8.93%) were the dominant genera.Alternaria mainly existed in 2-year-old twig and scapus.Anthina and Ectostroma existed in 2-year-old twig.Among different parts of the seedlings,the diversity of fungal endophytes was maximum in 1-yesr-old twig with Shannon-Wiener index 1.07,among different habitats,seedlings growing in Shanyang exhibited the highest number of fungal endohpytes in spring with Shannon-Wiener index of 1.30,The results suggested that endophytic fungi from J.regia in spring were rich resources for use. |
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| Keywords: | Juglans regia different habitat endophytic fungus biodiversity |
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