| 栓皮栎成熟效应蛋白质及组分变化分析 |
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| 引用本文： | 孙琳,张存旭.栓皮栎成熟效应蛋白质及组分变化分析[J].西北林学院学报,2016,31(3):114-118. |
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| 作者姓名： | 孙琳 张存旭 |
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| 作者单位： | (西北农林科技大学 林学院,陕西 杨陵 712100) |
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| 摘 要： | 选用幼龄、成龄、老龄栓皮栎在幼年期和成年期的叶片，研究成熟效应过程中蛋白质及组分变化规律。结果表明，蛋白质含量随成熟效应呈现由高到低的变化趋势为幼年期﹥成年期，幼龄﹥成龄﹥老龄；不同蛋白质组分在成熟效应过程中变化动态和含量都存在差异，清蛋白含量逐渐增加，出现了27.0、61.8 kD与104.8 kD的清蛋白条带，球蛋白、醇溶蛋白含量逐渐减少，14.4、28.4、53.4 kD与66.0 kD的球蛋白条带和53.4 kD的醇溶蛋白条带消失，谷蛋白含量无明显变化，4种蛋白组分含量表现为清蛋白>球蛋白>醇溶蛋白>谷蛋白。
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| 关 键 词： | 栓皮栎 蛋白质提取 成熟效应 SDS-PAGE |
Protein and Its Component Analysis in the Maturation of Quercus variabilis |
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| SUN Lin,ZHANG Cun-xu. Protein and Its Component Analysis in the Maturation of Quercus variabilis[J].Journal of Northwest Forestry University,2016,31(3):114-118. |
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| Authors: | SUN Lin ZHANG Cun-xu |
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| Institution: | (College of Forestry,Northwest A&F University,Yangling,Shaanxi 712100,China) |
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| Abstract: | The leaves which were in juvenile and matured growth stages were collected from 20- (young),40- (matured) and 70-year-old (aged) Quercus variabilis trees to examine the changes of protein contents and components.The content of protein in juvenile stage was higher than that in matured stage,and the order of protein contents for the leaves from different tree ages was young>matured>aged.Differences in protein content and composition during the maturation were observed.The content of albumin gradually increased,new albumin bands were found in the bands of 27.0 kD,61.8 kD,and 104.8 kD during maturation.Gliadin and globulin contents decreased,and the gliadin bands of 14.4 kD,28.4 kD,53.4 kD,66.0 kD and the globulin bands of 53.4 kD disappeared.Glutenin content had no significant change.The contents of four protein components were albumin>globulin>gliadin>glutenin. |
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| Keywords: | Quercus variabilisQuercus variabilis protein extraction maturation SDS-PAGE |
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