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不同处理对毛竹伐后竹篼微生物数量与酶活性的影响
引用本文:李美群,孟勇,杨明,艾文胜,涂佳,胡伟,肖飞.不同处理对毛竹伐后竹篼微生物数量与酶活性的影响[J].西北林学院学报,2016,31(3):148-153.
作者姓名:李美群  孟勇  杨明  艾文胜  涂佳  胡伟  肖飞
作者单位:(湖南省林业科学院,湖南 长沙 410004)
摘    要:采用促腐剂、机械破篼、覆盖等方式对毛竹林中新砍伐后的竹篼进行促腐处理,研究不同处理对毛竹伐后竹篼微生物数量及酶活性的影响。结果表明,不同处理的毛竹伐后竹篼微生物数量以细菌>纤维素菌>木质素菌>放线菌>真菌,微生物数量的对数值最高分别为10.28、10.01、9.11、8.77、8.47。酶活性以棉花酶>滤纸酶>CMC酶,锰过氧化物酶>漆酶>木质素过氧化氢酶,其最高分别为260.97、112.89、49.89、64.73、3.81 U·kg-1和0.069 U·kg-1。放线菌数量与真菌数量,纤维素菌数量与真菌、放线菌数量存在极显著正相关;滤纸酶活性与木质素菌数量、CMC酶活性与棉花酶活性存在显著正相关。以纤维素菌和木质素菌数量为正交指标的最佳组合为添加液态菌剂、破篼机破碎、保水剂和枯落物覆盖。采用TOPSIS优劣解距离法对微生物和酶活性进行综合评价,得出相对接近程度值(Ci)最大的3个处理为3号、7号、5号,数值分别为0.806 2、0.782 1、0.744 9,3个样地的Ci值相差不大,处理方法可用于毛竹伐后竹篼促腐。

关 键 词:毛竹篼  处理  微生物数量  酶活性

 Effects of Different Decay Promotion Treatments on Microorganism and Enzyme Activities of Bamboo Stump after Cut
LI Mei-qun,MENG Yong,YANG Ming,AI Wen-sheng,TU Jia,HU Wei,XIAO Fei. Effects of Different Decay Promotion Treatments on Microorganism and Enzyme Activities of Bamboo Stump after Cut[J].Journal of Northwest Forestry University,2016,31(3):148-153.
Authors:LI Mei-qun  MENG Yong  YANG Ming  AI Wen-sheng  TU Jia  HU Wei  XIAO Fei
Institution:(Hunan Academy of Forestry,Changsha,Hunan 410004,China)
Abstract:Promoted decay was conducted on newly cut bamboo stump by the methods of applying reagents,mechanical treatment,and covering to study their effects on microbes and enzyme activities.The results showed after treated by different methods,the number of microbes was in the order of bacteria > cellulose bacteria > lignin bacteria > actinomycetes > fungi,the corresponding highest logarithmic values of the microbes were 10.28,10.01,9.11,8.77,8.47,respectively.The enzymic activities were in the order of cotton enzymey > filter paper enzyme > CMC enzyme,manganese peroxidase enzyme > laccase enzyme > lignin catalase enzyme,with the corresponding highest values of enzyme activities of 260.97,112.89,49.89,64.73,3.81,and 0.069 U·kg-1,respectively.Significant positive correlations were observed between the amount of actinomycetes and fungi,cellulose bacteria and fungi & actinomycetes.Filter paper enzyme activity and the amount of lignin bacteria,CMC enzyme activity and cotton enzyme activity were significantly and positively correlated.Taking the amount of cellulose bacteria and lignin bacteria as orthogonal indicators,it was found that the best combination was adding liquid bacteria agent,stump broken treatment,water retention agent and litter cover processing.Using TOPSIS pros and cons solutions distance method on microbial and enzyme activity for integrated evaluation,the three biggest processing of Ci value were No.3,7,and 5,with numerical values of 0.806 2,0.782 1,and 0.744 9,respectively.The difference of Ci values was small among the three treatments,which could be used in promoting the decay process of bamboo stump after cut.
Keywords:bamboo stump  treatment  microorganism  enzyme activity
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