| 蛋白质凝胶电泳的高灵敏度染色法——复合银染 |
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| 引用本文： | 柳荣,黄建英,刘田生.蛋白质凝胶电泳的高灵敏度染色法——复合银染[J].天津农学院学报,1999(2). |
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| 作者姓名： | 柳荣 黄建英 刘田生 |
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| 作者单位： | 天津农学院动物科学系，天津市肿瘤研究所生物化学研究室 |
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| 摘 要： | 本实验以牛血清白蛋白为样品进行聚丙烯酰胺凝胶电泳，然后用考马斯亮兰Ｒ－２５０和银染法分步染色。这种复合银染法的灵敏度比单独使用银染法提高约１０００倍，比单独使用考马斯亮兰Ｒ－２５０染色法提高约１０６倍。它在１ｍｍ厚的聚丙烯酰胺凝胶上至少可检测出５ｐｇ／ｍｍ２的蛋白质。在没有放射性防护设施的任何一个生物实验室中，采用复合银染法基本上能够达到或接近放射性标记检测的灵敏度。相比之下，它不仅省去了繁琐的各式各样的标记过程及其放射性污染，同时还具有很高的灵敏度，因此复合银染具有广泛的应用性。
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| 关 键 词： | 蛋白质 聚丙烯酰胺凝胶电泳 银染 复合银染 染色 灵敏度 |
A Highly Sensitive Silver Stain for Proteins after\=Polyacrylamide Gel Electrophoresis |
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| Liu Rong,Huang Jianying,Liu Tiansheng.A Highly Sensitive Silver Stain for Proteins after\=Polyacrylamide Gel Electrophoresis[J].Journal of Tianjin Agricultural College,1999(2). |
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| Authors: | Liu Rong Huang Jianying Liu Tiansheng |
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| Institution: | Liu Rong1,Huang Jianying2,Liu Tiansheng1 |
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| Abstract: | Bovine serum albumin was used as sample to carry out polyacrylamide gel electrophoresis and then was stained by using a complex silver stain that included coomassie blue stain and silver stain. The sensitivity of this complex silver stain was 1 000 times higher than silver stain and 106 times higher than coomassie blue stain. At least 5 pg/mm2 protein in gel could be detected. The sensitivity of radioactive label could be reached by the complex silver stain. So the complex silver stain could be a very useful method.\= |
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| Keywords: | Protein Polyacrylamide gel electrophoresis Silver stain Complex silver stain Sensitivity |
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