| 西伯利亚蓼PsLEA基因的克隆及在NaHCO_3胁迫下的表达 |
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| 引用本文： | 李扬,刘关君,曲春浦,杨春雪,魏志刚,刘桂丰,杨传平.西伯利亚蓼PsLEA基因的克隆及在NaHCO_3胁迫下的表达[J].分子植物育种,2010,8(2):276-282. |
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| 作者姓名： | 李扬 刘关君 曲春浦 杨春雪 魏志刚 刘桂丰 杨传平 |
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| 作者单位： | 1. 东北林业大学林木遗传育种与生物技术国家教育部重点实验室,哈尔滨,150040
2. 东北林业大学林木遗传育种与生物技术国家教育部重点实验室,哈尔滨,150040;东北林业大学林术遗传育种黑龙江省重点实验室,哈尔滨,150040 |
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| 基金项目： | 教育部科学技术研究重点项目 |
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| 摘 要： | 根据西伯利亚蓼叶片cDNA文库中获得的胚胎发育晚期丰富蛋白(late embryogenesis abundant proteins,LEA)基因的部分序列,采用cDNA末端快速扩增(RACE)技术成功克隆了胚胎发育晚期丰富蛋白基因(LEA),命名为PsLEA(GenBank登录号:FJ478172)。该基因全长686bp,5'非翻译区为102bp,3'非翻译区为131bp,开放读码框为453bp,编码150个氨基酸。荧光定量PCR分析表明:正常情况下PsLEA基因在西伯利亚蓼的叶、茎和地下茎中皆有表达,其中茎中表达量最高。3%NaHCO3诱导胁迫下,叶、茎、地下茎中PsLEA表达均受到影响且表达模式存在差异,说明PsLEA基因与西伯利亚蓼的耐盐性相关。
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| 关 键 词： | 西伯利亚蓼 胚胎发育晚期丰富蛋白 cDNA末端快速扩增(RACE) 荧光定量PCR |
Cloning of PsLEA Gene from Polygonum sibiricum Laxm.and its Expression under NaHCO3 Stress |
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| Li Yang,Liu Guanjun,Qu Chunpu,Yang Chunxue,Wei Zhigang,Liu Guifeng,Yang Chuanping.Cloning of PsLEA Gene from Polygonum sibiricum Laxm.and its Expression under NaHCO3 Stress[J].Molecular Plant Breeding,2010,8(2):276-282. |
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| Authors: | Li Yang Liu Guanjun Qu Chunpu Yang Chunxue Wei Zhigang Liu Guifeng Yang Chuanping |
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| Abstract: | Based on a partial sequence of LEA (late embryogenesis abundant proteins) gene from cDNA library of Polygonum sibiricum Laxm.leaves,the LEA gene,designated as PsLEA (GenBank accession No.FJ478172.),was cloned by using rapid amplification of cDNA end (RACE) technology,The acquired gene was 686 bp in full length,including 102 bp of 5' untranslated region and 131 bp of 3' untranslated region.The open reading frame (ORF) is 453 bp in length,encoding a deduced amino acid sequence of 150 residues.Quantitative fluorescent PCR (QF-PCR) results show that PsLEA gene was expressed in leaves and stems as well as underground stems of Polygonum sibiricum Laxm.under normal conditions,and the expression level was higher in stems than in leaves and underground stems.Under 3% NaHCO3 stress,the expression level is greatly affected and the expression patterns varied in different tissues,implying that PsLEA gene plays a role in the salt resistance of Polygonum sibiricum Laxm.. |
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| Keywords: | Polygonum sibiricum Laxm Late embryogenesis abundant protein (LEA) Rapid amplification of cDNA end (RACE) Quantitative fluorescent PCR (QF-PCR) |
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