| 16个天然冰草种群遗传多样性RAPD分析 |
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| 引用本文： | 李景欣,云锦凤,郭军.16个天然冰草种群遗传多样性RAPD分析[J].草地学报,2005,13(3):190-193. |
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| 作者姓名： | 李景欣 云锦凤 郭军 |
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| 作者单位： | 1. 内蒙古民族大学, 通辽, 028042;2. 内蒙古农业大学, 呼和浩特, 010019 |
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| 基金项目： | 国家高技术研究发展计划(863计划) |
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| 摘 要： | 采用57个10 bp随机引物,对16个天然冰草(Agropyron cristatum)种群352个单株的基因组DNA进行RAPD多态性检测。结果表明:从57个RAPD引物中选出多态性标记的引物11个;检测出125个位点,102个显多态性;用Shan-non多样性指数量化的遗传多态度为2.19,种群内和种群间遗传变异比例分别为60%和40%,种群间的遗传相似度在0.7702~0.9776之间,遗传距离的变化范围为0.0226~0.2611;聚类分析结果表明:16个种群大致可分为4类,生境和表型相近的种群基本聚为一类,和形态学研究的结果基本一致,证明物种变异与生境密切相关。
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| 关 键 词： | 冰草 遗传多样性 遗传相似度 遗传距离 聚类分析 随即扩增多态DNA 种群 |
| 文章编号： | 1007-0435（2005）03-0190-04 |
| 收稿时间： | 2004-05-08 |
| 修稿时间： | 2004-12-25 |
RAPD Analysis of the Genetic Diversity of 16 Natural Wheatgrass Populations (Agropyron Cristatum ) |
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| LI Jing-xin,YUN Jin-feng,GUO Jun.RAPD Analysis of the Genetic Diversity of 16 Natural Wheatgrass Populations (Agropyron Cristatum )[J].Acta Agrestia Sinica,2005,13(3):190-193. |
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| Authors: | LI Jing-xin YUN Jin-feng GUO Jun |
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| Institution: | 1. Agronomy College, Inner Mongolia Nationalities University, Tongliao, Inner Mongolia Autonomous Region 028042, China;2. Ecology and Environmental Science College, Inner Mongolia Agricultural University, Huhhot, Inner Mongolia Autonomous Region 010019, China |
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| Abstract: | Eleven random primers (10bp)were selected from 57 primers for RAPD analysis of 352 wheatgrass individuals which were collected from 16 natural populations of Inner Mongolia. A hundred and three polymorphic loci were detected from 125 loci. Calculated by Shannon Index, the average genetic diversity was 2.19, the percentage of genetic variation were 60% within each population and 40% among the populations. The interpopulation similarity range was 0.7702～0.9776 and the interpopulation genetic distance range was (0.0226～0.2611). Through cluster analysis, the 16 populations were divided into 4 categories. The populations with similar ecological environment and prototype clustered together, a fact basically consistent with the result of morphological research, indicating that species variation is closely related to the environment. |
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| Keywords: | Agropyron cristatum Genetic diversity Genetic similarity Genetic distance Cluster analysis RAPD(Random Amplified Polymorphic DNA ) Population |
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