| 枯草芽孢杆菌BS-2对脉孢菌的抑菌活性成分分析 |
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| 引用本文： | 吴小平,彭建升,谢宝贵,柯斌榕,胡方平.枯草芽孢杆菌BS-2对脉孢菌的抑菌活性成分分析[J].热带作物学报,2010,31(11):1991-1995. |
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| 作者姓名： | 吴小平 彭建升 谢宝贵 柯斌榕 胡方平 |
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| 作者单位： | 1. 福建林大学生命科学学院,福建福州,350002;福建农林大学菌物研究中心,福建福州,350002
2. 福建农林大学植物保护学院,福建福州,350002 |
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| 摘 要： | 枯草芽孢杆菌(Bacillus subtilis)BS-2对脉孢菌(Neurospora)有较强的抑制作用。本文通过对枯草芽孢杆菌BS-2进行紫外诱变,获得了抑菌活性比出发菌株增强的突变株BS-2-E和抑菌活性完全丧失的突变株BS-2-N。用LC-MS分析BS-2、BS-2-E及BS-2-N发酵液的盐酸粗提物,比较这3个菌株的质谱响应峰,初步认为BS-2的抑菌活性物质主要是伊枯草菌素(iturins)和芬枯草菌素B(fengycin B)。
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| 关 键 词： | 枯草芽孢杆菌 生物防治 脉孢菌 食用菌 |
The Analysis of Antifungal Activity Composition of Bacillus subtilis BS-2 |
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| Wu Xiaoping,Peng Jiansheng,Xie Baogui,ke Binrong and Hu Fangping.The Analysis of Antifungal Activity Composition of Bacillus subtilis BS-2[J].Chinese Journal of Tropical Crops,2010,31(11):1991-1995. |
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| Authors: | Wu Xiaoping Peng Jiansheng Xie Baogui ke Binrong and Hu Fangping |
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| Institution: | 1 College of Life Science, Fujian Agriculture and Forestry University2 Mycological Research Center, Fujian Agriculture and Forestry University;1 College of Life Science, Fujian Agriculture and Forestry University2 Mycological Research Center, Fujian Agriculture and Forestry University;1 College of Life Science, Fujian Agriculture and Forestry University2 Mycological Research Center, Fujian Agriculture and Forestry University;1 College of Life Science, Fujian Agriculture and Forestry University2 Mycological Research Center, Fujian Agriculture and Forestry University;College of Plant Protection, Fujian Agriculture and Forestry University |
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| Abstract: | Bacillus subtilis BS-2 could inhibit the growth of Neurospora. By ultraviolet mutagenesis, two mutant strains, namely BS-2-E and BS-2-N, were obtained. The inhibiting activity of the mutant strain BS-2-E on Neurospora was obviously increased, but the mutant strain BS-2-N was not any inhibiting activity on Neurospora. Based on the Mass spectrum analysis, the active substances in the strain BS-2 were Ituins and Fengycin B. |
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| Keywords: | LC-MS |
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