| 草莓外源LEA3基因的导入 |
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| 引用本文： | 王俊丽,葛会波,彭士琪,张红梅,续九如.草莓外源LEA3基因的导入[J].园艺学报,2003,30(3):322-324. |
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| 作者姓名： | 王俊丽 葛会波 彭士琪 张红梅 续九如 |
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| 作者单位： | ( 河北大学生命科学学院，保定071002； 北京林业大学生物科学与技术学院，北京100083； 河北农业大学园艺学院，保定071001) |
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| 摘 要： | 以草莓(Fragaria ananassa Duch．)品种‘Darslect’的花药为试材，以MS培养基为基本培养基，进行愈伤组织的诱导。通过基因枪法将来源于大麦的胚胎发生晚期丰富蛋白基因LEA3 导入愈伤组织细胞，经过除草剂PFF的4次筛选培养，获得了抗性愈伤组织及再生植株。通过地高辛标记的探针进行Southern杂交分析，检测到了杂交带，表明LEA3 基因整合到草莓染色体基因组中。
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| 关 键 词： | 草莓 花药 愈伤组织 LEA3 蛋白基因 转化 |
| 文章编号： | 0513-353X(2003)03-0322-03 |
| 收稿时间： | 2002-6-17 |
| 修稿时间： | 2002年6月17日 |
LEA3 Gene Transferred into Strawberry |
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| Wang Junli ,Ge Huibo ,Peng Shiqi ,Zhang Hongmei ,and Xu Jiuru.LEA3 Gene Transferred into Strawberry[J].Acta Horticulturae Sinica,2003,30(3):322-324. |
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| Authors: | Wang Junli Ge Huibo Peng Shiqi Zhang Hongmei and Xu Jiuru |
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| Institution: | ( Colege of Science，Hebei University，Baoding 071002，China; College of Biological Science and Technology,Beijing Forestry University, 100083, China; College of Horticulture，Hebei Agricultural University，Baoding 071001,China) |
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| Abstract: | Callus of anther from strawberry(Fragaria ananassa Duch.Darslect)was induced on MS medium. Late embryogenesis abundant protein gene,LEA 3,from barley(Hordeum vulgare L.)was introduced into strawberry callus by the aid of biolistic-mediated transformation method.After 4 times of PPT selected culture,resistant calli and regenerated plants were obtained.The hybridization bands were determined in different transgenic plants by southern blot,and this showed that LEA 3 gene was integrated into strawberry genome. |
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| Keywords: | Strawberry Anther Callus LEA 3 gene Transformation |
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