| PGM-35Sbar玉米通用表达载体的构建及玉米转化研究(英文) |
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| 引用本文： | 王霄汉,罗昌,程曦,张秀海,黄丛林,吴忠义.PGM-35Sbar玉米通用表达载体的构建及玉米转化研究(英文)[J].农业科学与技术,2012(8):1679-1683. |
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| 作者姓名： | 王霄汉 罗昌 程曦 张秀海 黄丛林 吴忠义 |
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| 作者单位： | 首都师范大学生命科学学院;北京市农林科学院,北京农业生物技术研究中心 |
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| 基金项目： | Supported by National Transgenic Special Project(2009ZX08003-009B);Beijing Science and Technology Commission Project(KJCX201102003)~~ |
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| 摘 要： | 目的]构建玉米通用表达载体,以期为利用转基因手段提高玉米对非生物胁迫的耐受性奠定基础。方法]通过对现有的pGreen0229植物表达载体进行改造,构建含有CaMv35S启动子驱动的抗草胺膦筛选基因bar、可用于连接目的基因的玉米表达载体PGM-35Sbar,并通过花粉管通道法转化吉444玉米自交系。结果]PGM-35Sbar玉米通用载体构建成功,转化玉米植株后,得到抗性植株14棵,经PCR检测其中有12棵为阳性植株。结论]该研究为快速构建含有特定目的基因的玉米表达载体奠定了基础。
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| 关 键 词： | 玉米通用表达载体 花粉管通道法 除草剂抗性 转基因玉米 |
Construction of Maize Universal Expression Vector PGM-35Sbar and Maize Transformation |
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| Xiaohan WANG,Chang LUO,Xi CHENG,Xiuhai ZHANG,Conglin HUANG,Zhongyi WU.Construction of Maize Universal Expression Vector PGM-35Sbar and Maize Transformation[J].Agricultural Science ＆ Technology,2012(8):1679-1683. |
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| Authors: | Xiaohan WANG Chang LUO Xi CHENG Xiuhai ZHANG Conglin HUANG Zhongyi WU |
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| Institution: | 1,2*1. College of Life Science, Capital Normal University, Beijing 100048, China; 2. Beijing Agro-Biotechnology Research Center, Beijing Academy of Agriculture and Forestry Sciences, Beijing 100097, China |
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| Abstract: | Objective] The paper was to construct maize universal expression vector, in order to provide basis for using transgenic methods to improve abiotic stress tolerance of maize. Method] Based on the transformation of existing pGreen0229 plant expression vector, phosphinothricin-resistant selectable marker-bar gene driving by CaMv35S promoter was constructed, which could be used to connect target gene of maize expression vector PGM-35Sbar, and transform Ji444 maize inbred lines by pollen tube pathway. Result] The universal expression vector for PGM-35Sbar maize had been successfully constructed. When the maize plants were transformed, 14 resistant plants were obtained, and 12 plants were identified to be positive plants by PCR detection. Conclusion] The study provided basis for rapid construction of maize expression vector containing specific target gene. |
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| Keywords: | Maize universal expression vector Pollen tube pathway Herbicide resistance Transgenic maize |
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