| 乳汁中RNA提取方法的建立 |
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| 引用本文： | 乔新安,朱彦彩,范沛,都军霞,贾海丽,韩立强,杨国字.乳汁中RNA提取方法的建立[J].中国畜牧兽医,2008,35(1):77-79. |
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| 作者姓名： | 乔新安 朱彦彩 范沛 都军霞 贾海丽 韩立强 杨国字 |
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| 作者单位： | 1河南农业大学动物生理生化实验室，郑州 450002；2河南科技学院细胞工程重点实验室，新乡 453003 |
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| 基金项目： | 河南省重点科技攻关项目(0523010500) |
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| 摘 要： | 用牛初乳作为试验材料，进行乳汁体细胞的提取及RNA的提取与扩增。结果显示：牛初乳中含有大量的体细胞（SCC约为20万/ml，活力约为60%），从体细胞中提取RNA，所提取的RNA完整性和纯度可以用来合成cDNA，管家基因GAPDH扩增条带正确，表明可以用合成的cDNA作为模板进行其它基因的扩增，为奶牛在基因工程方面的研究提供分子生物学技术平台。
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| 关 键 词： | 牛初乳 乳汁体细胞 RNA提取 |
| 文章编号： | 1671-7236（2008）01-0077-03 |
| 收稿时间： | 2007-07-20 |
| 修稿时间： | 2007年7月20日 |
The Establishment of the Method of the Extraction of RNA from Milk |
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| QIAO Xin-an,ZHU Yan-cai,FAN Pei,DU Jun-xia,JIA Hai-li,HAN Li-qiang,YANG Guo-yu.The Establishment of the Method of the Extraction of RNA from Milk[J].China Animal Husbandry & Veterinary Medicine,2008,35(1):77-79. |
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| Authors: | QIAO Xin-an ZHU Yan-cai FAN Pei DU Jun-xia JIA Hai-li HAN Li-qiang YANG Guo-yu |
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| Institution: | 1Lab of Animal Physiology & Biochemistry, Henan Agricultural University, Zhengzhou 450002,China;2Key Laboratory of Cell Engineering, Henan Institute of Science and Technology, Xinxiang 453003,China |
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| Abstract: | The cow colostrum was applied as the experimental material,and the milk somatic cells were extracted and RNA was extracted and amplicated.The result shows that the colostrum contains a large number of somatic cells(SCC is about 200,000 /ml and the activity of somatic cells is about 60%).The integrality and purity quotient of the extracted RNA can be used to produce DNA.By RT-PCR amplication,the bands of house-keeping gene GAPDH was reasonable,which indicates that produced DNA can be amplificated as the template for other genes.It provides the primary basis of molecular biological analysis for the cow in the aspect of the genetic engineering research. |
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| Keywords: | cow colostrums somatic cell from milk the extraction of RNA |
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