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Development of molecular markers based on retrotransposons for the analysis of genetic variability in Moniliophthora perniciosa |
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| Authors: | Mateus Ferreira Santana Elza Fernandes de Araújo Jorge Teodoro de Souza Eduardo Seiti Gomide Mizubuti Marisa Vieira de Queiroz |
| Institution: | 1. Departamento de Microbiologia, Instituto de Biotecnologia Aplicada a Agropecu??ria (BIOAGRO), Universidade Federal de Vi?osa, 36570-000, Vi?osa, MG, Brazil 2. Centro de Ci??ncias Agr??rias, Biol??gicas e Ambientais, Universidade Federal do Rec?ncavo da Bahia, 44380-000, Cruz das Almas, BA, Brazil 3. Departamento de Fitopatologia, Universidade Federal de Vi?osa, 36570-000, Vi?osa, MG, Brazil |
| Abstract: | Moniliophthora perniciosa is a fungus that causes witches?? broom disease (WBD) in the cacao tree (Theobroma cacao). The M. perniciosa genome contains different transposable elements; this prompted an evaluation of the use of its retrotransposons as molecular markers for population studies. The inter-retrotransposon amplified polymorphism (IRAP) and retrotransposon-microsatellite amplified polymorphism (REMAP) techniques were used to study the variability of 70?M. perniciosa isolates from different geographic origins and biotypes. A total of 43 loci was amplified. Cluster analysis of different geographical regions of C biotype revealed two large groups in the state of Bahia, Brazil. Techniques using retrotransposon-based molecular markers showed advantages over previously used molecular techniques for the study of genetic variability in M. perniciosa. |
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