Sequence Analysis Shows that a Dwarfing Disease on Rice, Wheat and Maize in China is Caused by Rice Black-streaked Dwarf Virus |
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| Authors: | Hengmu Zhang Jianping Chen Juanli Lei Michael J Adams |
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| Institution: | (1) College of Agronomy and Biotechnology, Zhejiang University, Hangzhou, 310029, China;(2) Virology Laboratory, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China;(3) Virology Laboratory, Zhejiang Academy of Agricultural Sciences, Hangzhou, 310021, China;(4) Plant Pathology Department, IACR-Rothamsted, Harpenden, Herts, AL5 2JQ, UK |
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| Abstract: | Isolates of plant reoviruses causing severe stunting and dark leaf symptoms on wheat in Hebei province, on maize in Hubei province and on rice in Zhejiang province, China have been characterized. Four of the ten genome segments corresponding to Rice black-streaked dwarf virus (RBSDV) S7, S8, S9, S10 were amplified by RT-PCR from the Hebei and Zhejiang isolates and sequenced. Sequences of S9 and S10 were also obtained from the Hubei isolate. Sequences of corresponding segments of the Chinese isolates were very similar to each other (94.0–99.0% identical nucleotides and 96.3–100% identical amino acids) and were closer to those of a previously reported Japanese RBSDV isolate (90.0–94.9% identical nucleotides and 91.1–98.6% identical amino acids) than to those of an Italian Maize rough dwarf virus isolate (85.1–88.1% identical nucleotides and 85.5–94.3% identical amino acids). The Chinese isolates should be classified as RBSDV. |
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| Keywords: | Fijivirus Maize rough dwarf virus homology |
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