| 枯草芽孢杆菌Bs-15产嗜铁素条件及其对甜椒的防病促生效应 |
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| 引用本文： | 余贤美,周广芳,辛力.枯草芽孢杆菌Bs-15产嗜铁素条件及其对甜椒的防病促生效应[J].农药学学报,2010,12(2):135-141. |
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| 作者姓名： | 余贤美 周广芳 辛力 |
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| 作者单位： | 1.山东省果树研究所,山东 泰安 271000 |
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| 基金项目： | "十一五"国家科技支撑计划资助项目 |
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| 摘 要： | 研究了不同培养条件对枯草芽孢杆菌Bs-15嗜铁素产量的影响。结果表明,以改良MM培养基为基础培养基,以葡萄糖为碳源,以色氨酸为外源氨基酸,在pH 7.2、装液量100 mL/250 mL、37℃、200 r/min下培养,可获得较高产量的嗜铁素。盆钵试验结果表明:若同时接种 Bs-15和枯萎病菌,Bs对枯萎病菌的防效为44.44%;若施用Bs-15 2 d后再接种枯萎病菌,防效可达56.94%。说明Bs-15可能通过产生系统诱导抗性增强了对枯萎病的防效。研究结果还表明,Fe离子可大大降低Bs-15对枯萎病的防治效果,防效仅为12.50%和23.43%。另外,经Bs-15浸种处理播种后第14,21,28和40 d的甜椒苗比对照增高27.24% ~54.53%;与对照相比,处理组中有50%的甜椒开花时间提前了17.26 d,平均单果重和单株产量分别提高36.92%和49.68%。表明枯草芽孢杆菌Bs-15在甜椒枯萎病防治及生长促进作用方面具有较好的效果。
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| 关 键 词： | 枯草芽孢杆菌 甜椒 镰刀菌枯萎病 生物防治 植物促生作用 |
| 收稿时间： | 2/1/2010 12:00:00 AM |
| 修稿时间： | 2010/3/30 0:00:00 |
Study on factors influencing the siderophore production ofBacillus subtilis Bs-15 and effects of Bs-15 on diseasecontrol and growth promotion of sweet pepper |
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| YU Xian-mei,ZHOU Guang-fang and XIN Li.Study on factors influencing the siderophore production ofBacillus subtilis Bs-15 and effects of Bs-15 on diseasecontrol and growth promotion of sweet pepper[J].Chinese Journal of Pesticide Science,2010,12(2):135-141. |
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| Authors: | YU Xian-mei ZHOU Guang-fang and XIN Li |
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| Institution: | 1.Shandong Institute of Pomology,Tai'an 271000,Shandong Province,China |
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| Abstract: | The influence of culture conditions on the siderophore production of Bacillus subtilis Bs-15 was studied.The results showed that Bs-15 could yield high content of siderophore when inoculated 100 mL modified MM medium (pH 7.2) with glucose as carbon source and tryptophan as nitrogen source,and shaking under 200 r/min in 250 mL flask at 37℃.The effects of B.subtilis Bs-15 on the biocontrol of Fusarium wilt and growth promotion of sweet pepper was studied by pot experiment.The results showed that Bs-15 decreased the incidence of Fusarium wilt significantly,with the control efficacy of 44.4% when inoculated B.subtilis Bs-15 and Fusarium oxysporum Schl.f.sp. simultaneously,and of 56.94% when inoculated F.oxysporum Schl.f.sp.2 days after treatment of B.subtilis Bs-15;However,iron significantly reduced the suppression effect of Bs-15 on Fusarium wilt of sweet pepper,with control efficacy of 12.5% and 23.4% ,respectively.There were significantly differences between Bs-15 treatment and control in plant height after treatment for 14,21,28 and 40 days,which increased from 27.24% to 54.53%.B.subtilis Bs-15 could shorten the days of 50 percent flowering of sweet pepper by 17.26 days,and enhance the average fruit weight and yield per plant of sweet pepper by 36.92% and 49.68%,respectively.The results indicated that B.subtilis Bs-15 had good effects on the Fusarium wilt and growth promotion of sweet pepper. |
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| Keywords: | Bacillus subtilis sweet pepper (Capsicum annuum L ) Fusarium wilt biological control plant growth promotion |
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