| 保存在重铬酸钾中的微小隐孢子虫卵囊活性及传染性研究 |
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| 引用本文： | 陈甫,黄克和.保存在重铬酸钾中的微小隐孢子虫卵囊活性及传染性研究[J].中国农学通报,2009,25(7):13-17. |
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| 作者姓名： | 陈甫 黄克和 |
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| 作者单位： | 1. 南京农业大学动物医学院,南京,210095;青岛农业大学动物科技学院,山东青岛,266109
2. 南京农业大学动物医学院,南京,210095 |
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| 基金项目： | 国家自然科学基金，高等学校博士学科点专项科研基金，国家重点实验室开放基金 |
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| 摘 要： | 目的 探索保存在4℃ 2.5 %重铬酸钾(K2Cr2O7)中1－30个月的微小隐孢子虫卵囊（CPO）的活性和对免疫抑制BALB/C小鼠的感染性。方法 通过体外脱囊技术测定卵囊的脱囊率评价卵囊的活性；以BALB/C小鼠为感染模型，通过检测CPO感染小鼠的潜伏期、排卵囊数量和回肠末端绒毛中的隐孢子虫数量来评价卵囊的传染性。结果 保存在2.5 % K2Cr2O7中1—16个月的卵囊出现脱囊；免疫抑制BALB/C小鼠在感染保存1—16个月的卵囊后3－8 d开始排出大量的卵囊，且发现在末端回肠绒毛中寄生有大量的隐孢子虫；卵囊的保存时间与潜伏期之间存在强烈的相关性(r2＝0.92)。结论CPO在4℃ 2.5 % K2Cr2O7中能保持活性和传染性至少16个月，K2Cr2O7是保存CPO的良好介质。
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| 关 键 词： | RNA干扰 RNA干扰 双链RNA 基因功能 应用 |
| 收稿时间： | 2008-12-25 |
| 修稿时间： | 2009-02-12 |
Viability and infectivity of C. parvum Oocysts preserved in 2.5 percent of potassium dichromate |
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| Chen Fu,Huang Kehe.Viability and infectivity of C. parvum Oocysts preserved in 2.5 percent of potassium dichromate[J].Chinese Agricultural Science Bulletin,2009,25(7):13-17. |
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| Authors: | Chen Fu Huang Kehe |
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| Institution: | Chen Fu, Huang Kehe (1.College of Veterinary Medicine, Nanjing Agricultural University, Nanjing 210095; 2.College of Animal science and Veterinary Medicine, Qingdao Agricultural University, Qingdao Shandong 266109) |
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| Abstract: | Object: The present study was undertaken to compare the viability and infectivity of Cryptosporidium parvum oocysts (CPO) that had been stored for 1, 4, 7, 10, 13, 16, 20, 25 and 30 months at 4°C in 2.5% potassium dichromate (K2Cr2O7), respectively. Methods: An excystation protocol was performed in vitro to evaluate the viability of oocysts. One hundred and eighty female BABL/c mice were used to evaluate the infectivity of oocysts by detecting the prepatent period of C. parvum infection, the quantity of oocysts excreted, and the number of parasites that colonized the villi of the ileum. Results: CPO preserved in K2Cr2O7 for 1–16 months were capable of excystation in vitro and infection of mice in vivo. The prepatent period was extended from 3 to 8 days as the storage time of oocysts increased. There was a strong correlation between prepatent period and duration of oocyst storage (R2＝0.92). Large number of oocysts were isolated and purified from feces of mice inoculated oocysts preserved for 1-16 months in K2Cr2O7, respectively. Cryptosporidia parasitized in the terminal ilea were found in mice inoculated with oocysts stored in K2Cr2O7 for 1-16 months. Conclusion: CPO may be stored at 4°C in K2Cr2O7 for at least 16 months for the purposes of laboratory research, and K2Cr2O7 is a good medium of keeping viability and infectivity of CPO for a long time. |
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| Keywords: | potassium dichromate C parvum oocyst viability infectivity excystation in vitro |
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