| 冷冻干燥法制备速溶植酸酶的研究 |
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| 引用本文： | 马俊孝,刘伟,史衍鲁.冷冻干燥法制备速溶植酸酶的研究[J].饲料工业,2011(Z1). |
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| 作者姓名： | 马俊孝 刘伟 史衍鲁 |
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| 作者单位： | 康地恩生物集团,261500 |
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| 摘 要： | 采用毕赤酵母发酵制备酶液,膜超滤浓缩、硫酸铵盐析法沉淀酶蛋白,结合冷冻干燥法制得速溶型植酸酶。结果表明,对酶活力达19 500U/ml的发酵液进行浓缩后,以70%饱和度的硫酸铵进行盐析,分离后的酶蛋白经过冷冻干燥得到活力为500 000U/g的植酸酶,速溶性好,溶解后清亮透明。酶的冻干粉50℃保存90d内酶活力仍能维持96%以上的活力。为高酶活速溶植酸酶的工业化生产提供了一条可行的思路。
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| 关 键 词： | 植酸酶 速溶 盐析 冷冻干燥 |
Research on preparing instant phytase by using freeze-drying method |
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| Ma Junxiao,Liu Wei,Shi Yanlu.Research on preparing instant phytase by using freeze-drying method[J].Feed Industry,2011(Z1). |
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| Authors: | Ma Junxiao Liu Wei Shi Yanlu |
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| Institution: | Ma Junxiao,Liu Wei,Shi Yanlu |
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| Abstract: | The freeze-drying technology for producing instant phytase was studied.The crude preparation was obtained by Pichia pastoris high cell density fermentation,microporous membrane ultrafiltration, ammonium sulfate precipitation and freeze-drying.Fermentation liquid,activity achieved 19 500 U/ml, was managed by ultrafiltration membrane,concentrated with ammonium sulphate in 70%saturation.After freeze-drying,the final product was obtained with up to 500 000 U/g.The preparation could soluble in water quickly with... |
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| Keywords: | phytase instant powder ammonium sulphate precipitation freeze-drying |
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