| 利用 F2 转 Bt 基因抗虫杂交棉新策略 |
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| 引用本文： | 郭旺珍,孙敬,郭玉芳,张天真.利用 F2 转 Bt 基因抗虫杂交棉新策略[J].棉花学报,2001,13(4):200-203. |
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| 作者姓名： | 郭旺珍 孙敬 郭玉芳 张天真 |
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| 作者单位： | 南京农业大学遗传育种系,作物遗传与特异种质创新教育部重点实验室,农作物种质资源与育种农业部重点开放实验室,南京,210095 |
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| 摘 要： | 通过不同抗虫棉品系间互交、后代选择的方法选育出聚合整合位点不同的Bt基因纯合抗虫棉种质,开展了培育以 F2 利用为目的的杂交种探索研究.类似 F2 世代 Bt 基因的不同分离类型,聚合有不同数目 Bt 基因的棉株在整个生育期对棉铃虫有显著的抗虫性.并且与单价抗虫棉时空表达一致,生育前期抗虫性好,毒蛋白表达量高,中、后期抗虫性有所下降,毒蛋白表达量降低.因此,用其作为亲本配置抗虫杂交种,是利用棉花F2 杂种优势的途径之一.
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| 关 键 词： | 不同来源 Bt 基因 聚合 抗虫性 育种利用 |
| 文章编号： | 1002-7807(2001)04-0200-04 |
| 修稿时间： | 2000年12月22 |
A New Strategy to DevelopTransgenic Bt hybrid cotton of F2 Population |
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| GUO Wang zhen,SUN Jing,GUO Yu fang,ZhANG Tian zhen.A New Strategy to DevelopTransgenic Bt hybrid cotton of F2 Population[J].Cotton Science,2001,13(4):200-203. |
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| Authors: | GUO Wang zhen SUN Jing GUO Yu fang ZhANG Tian zhen |
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| Institution: | GUO Wang zhen,SUN Jing,GUO Yu fang,ZhANG Tian zhen * |
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| Abstract: | Researches were carried out to develop germplasm with two homozygous Bt genes inserted in different chromosomes by intercrossing between different transgenic Bt cotton strains for transgenic Bt F 2 hybrid cotton. The F 2 segregated out 1/16 insect susceptible plants using this germplasm as parents . Bioassy indicated that high resistance existed in 15/16 insect resistant plants although there were different dosages of inserted genes. Temporal difference of resistance in transgenic Bt plants was observed too, i.e., there was a declining level of efficacy with plant age as the mortality (%) of Helicoverpa armigera decreased gradually, when fed with leaves from the main stem. Our research suggested that it is reasonable to grow the transgenic F 2 hybrid crossed two homozygous Bt gene lines with commercial non transgenic Bt cotton varieties. |
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| Keywords: | upland cotton transgenic Bt F_2 hybrid heterosis gene resources |
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