| 禽白血病病毒囊膜基因gp85片段的克隆与鉴定 |
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| 引用本文： | 刘公平,刘福安,赵振芬.禽白血病病毒囊膜基因gp85片段的克隆与鉴定[J].中国预防兽医学报,2001,23(1):17-19. |
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| 作者姓名： | 刘公平 刘福安 赵振芬 |
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| 作者单位： | 1. 华南农业大学动物医学系,
2. 郑州牧业工程高等专科学校, |
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| 基金项目： | 高等学校博士学科点专项基金资助项目(编号为980501) |
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| 摘 要： | 用ＰＣＲ从禽白血病病毒（Avian Leukosis Virus,ALV)RAV-1,RAV-2感染或未感染的ＳＰＦ鸡胚成纤维细胞（ＣＥＦ）分别扩增出１．２kb囊膜基因片段，分别将上述ＰＣＲ产物的KpnⅠ／SaⅡ酶切片段克隆到质粒pGEM-3zf( )中得到３个重组子即pGEM-3zf-RAV-1env、pGEM-3zf-RAV-2env和pGEM-3zf-Eenv。酶切分析和序列测定结果表明克隆的ＰＣＲ片段分别来自ＡＬＶ ＲＡＶ－１，ＲＡＶ－２和内源生Ｅ亚群病毒，这为病毒囊膜基因gp85的表达及个体鸡遗传抗性的鉴定打下基础。
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| 关 键 词： | 禽白血病病毒 囊膜基因 克隆 gp85片段 |
| 文章编号： | 1008-0589(2001)01-0017-04 |
| 修稿时间： | 2000年6月26日 |
Cloning and Identification
of Envelope Gene gp85 of Avian Leukosis Virus |
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| LIU Gongping,Liu Fuan,ZHAO Zhenfen.Cloning and Identification
of Envelope Gene gp85 of Avian Leukosis Virus[J].Chinese Journal of Preventive Veterinary Medicine,2001,23(1):17-19. |
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| Authors: | LIU Gongping Liu Fuan ZHAO Zhenfen |
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| Institution: | LIU Gongping,ZHAO Zhenfen *,LIU Fuan |
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| Abstract: | kb PCR fragments were respectively obtained from SPF chicken embryo fibroblast cells (CEF),which were infected with Avian Leukosis Virus(ALV)RAV_1,RAV_2 or not with any ALV as control.These PCR products digested with KpnI and SalI were cloned into vector pGEM_3zf( ) respectively, three recombinant plasmids pGEM_3zf_RAV_1 env,pGEM_3zf_RAV_2 env, and pGEM_3zf_E env were obtained .The results of restriction analysis and sequence of the inserts of these three recombinant plasmids demonstrated that the cloned PCR fragments belonged to RAV_1,RAV_2,and E subgroup endogenous virus respectively.These provide a basis on the expressing of envelope gene gp85 of ALV and identificating the genetic resistant chicken against to ALV infection. |
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| Keywords: | Avian leukosis virus Envelope gene Cloning |
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